EXTRACELLULAR MATRIX METABOLISM BY CHONDROCYTES .5. PROTEOGLYCANS AND GLYCOSAMINOGLYCANS SYNTHESIZED BY CHONDROCYTES IN HIGH-DENSITY CULTURES
EXTRACELLULAR MATRIX METABOLISM BY CHONDROCYTES .5. PROTEOGLYCANS AND GLYCOSAMINOGLYCANS SYNTHESIZED BY CHONDROCYTES IN HIGH-DENSITY CULTURES
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DOI：
10.1016/0304-4165(79)90387-8
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发表时间：
1979-01-01
期刊：
影响因子：
--
通讯作者：
LOWTHER, DA
中科院分区：
文献类型：
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作者：
HANDLEY, CJ;LOWTHER, DA
从高密度培养的鸡胚软骨细胞的细胞外基质中提取蛋白多糖，并通过CsCl密度梯度离心纯化。蛋白多糖的化学、物理和透明质酸结合特性与在其他透明软骨蛋白多糖中观察到的相似。还纯化了培养基中的蛋白聚糖，分析显示存在3个蛋白聚糖群体。一个群体具有典型的蛋白聚糖亚基和结合透明质酸的物理特征，另外2个群体不能与透明质酸复合，但1个具有蛋白聚糖亚基的物理特征，另一个具有较小的MW。小分子量似乎是较大分子量物质酶促降解的产物。
Proteoglycans were extracted from the extracellular matrix of cultures of embryonic chick chondrocytes grown at high density and were purified by CsCl density gradient centrifugation. The chemical, physical and hyaluronate binding properties of the proteoglycans were similar to those observed in proteoglycans from other hyaline cartilages. Proteoglycans in the media were also purified and on analysis showed 3 populations of proteoglycans to be present. One population had the physical characteristics of a typical proteoglycan subunit and bound hyaluronate, the other 2 populations were unable to complex with hyaluronate but 1 had the physical characteristics of the proteoglycan subunit and the other was of smaller MW. The small MW appears to be a product of the enzymatic degradation of the larger MW species.