RT-PCR detection and sequence analysis of coat protein gene of Actinidia virus 1 from Sichuan Province.
RT-PCR detection and sequence analysis of coat protein gene of Actinidia virus 1 from Sichuan Province.
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DOI：
10.16420/j.issn.0513-353x.2019-0166
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发表时间：
2020-01-01
影响因子：
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通讯作者：
Xi, D. H.
中科院分区：
文献类型：
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作者：
Peng, Qi-ding;Lu, Rui;Xi, D. H.
最近，在新西兰报道了一种感染猕猴桃的新病毒，猕猴桃病毒1（Actinidia virus 1，AcV-1）。为了解四川猕猴桃AcV-1的发生情况及分子特征，采用RT-PCR方法对90份疑似感染AcV-1的猕猴桃叶片进行了检测。结果表明，AcV-1阳性22份，感染率为24.4%。将获得的5个AcV-1分离物的外壳蛋白基因（CP）序列与新西兰报道的分离物K75进行了比较。结果表明，这些分离物的CP核苷酸序列同源性为84.8%~ 97.1%，氨基酸序列同源性为89.7%~ 99.6%。其中，新西兰分离物K75与其他4个分离物的CP核苷酸序列同源性均小于91%，而琼来分离物HYH 5的同源性为97.1%。基于CP基因的系统发育分析表明，这些分离物主要聚为3个分支，其中HYH 5和新西兰分离物K75位于同一分支，其余分离物分别位于另外两个分支。
Recently, a new virus that infected kiwifruit, Actinidia virus 1 (AcV-1), has been reported in New Zealand. In order to determine the occurrence and molecular characterization of AcV-1 in kiwifruit cultivars ('Hongyang' and 'Jinguo') in Sichuan Province, 90 kiwifruit leaves samples of suspected viral infection were tested for AcV-1 by reverse transcription polymerase chain reaction (RT-PCR). The results showed that 22 samples were positive to AcV-1, and the infection rate was 24.4%. The coat protein gene (CP)sequences of the obtained five AcV-1 isolates were compared with isolate K75 which was reported in New Zealand. Results showed that the sequences identities of CP nucleotides and amino acid among these isolates were 84.8%-97.1% and 89.7%-99.6%, respectively. Among them, the sequence identities of CP nucleotides were less than 91% between New Zealand isolate K75 and four of these isolates except Qionglai isolate HYH5, which was 97.1%. Phylogenetic analysis based on CP gene of each isolate showed that these isolates were mainly clustered into three branches, HYH5 and New Zealand isolate K75 were located in the same branch, and the other isolates were located in the other two branches.