Effect of Hydrophobicity on Nano-Bio Interactions of Zwitterionic Luminescent Gold Nanoparticles at the Cellular Level.
Effect of Hydrophobicity on Nano-Bio Interactions of Zwitterionic Luminescent Gold Nanoparticles at the Cellular Level.
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DOI：
10.1021/acs.bioconjchem.8b00202
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发表时间：
2018-05
影响因子：
4.7
通讯作者：
Shasha Sun;Yingyu Huang;Chen Zhou;Sishan Chen;Mengxiao Yu;Jinbin Liu;Jie Zheng
中科院分区：
文献类型：
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作者：
Shasha Sun;Yingyu Huang;Chen Zhou;Sishan Chen;Mengxiao Yu;Jinbin Liu;Jie Zheng
基本了解疏水性如何影响工程纳米颗粒的细胞相互作用对于它们未来在医疗保健中的成功至关重要。在本文中，我们报告，插入疏水性辛硫醇到表面上的两性离子发光谷胱甘肽涂层的金纳米粒子（GS-AuNPs）的2 nm增强其亲和力的细胞膜和细胞摄取动力学增加了一个数量级以上，而不是诱导积累的AuNPs在双层核心或增强其被动扩散。这些研究突出了疏水性诱导的纳米生物相互作用在细胞水平上的多样性和异质性，并提供了一种新的途径来加速工程纳米颗粒的细胞摄取。此外，具有高亲和力的细胞膜和快速的内吞作用的两亲性发光金纳米粒子可能作为双模态成像探针，在细胞水平上关联荧光和电子显微镜。
Fundamental understanding of how the hydrophobicity impacts cellular interactions of engineered nanoparticles is critical to their future success in healthcare. Herein, we report that inserting hydrophobic octanethiol onto the surface of zwitterionic luminescent glutathione coated gold nanoparticles (GS-AuNPs) of 2 nm enhanced their affinity to the cellular membrane and increased cellular uptake kinetics by more than one order of magnitude, rather than inducing the accumulation of the AuNPs in the bilayer core or enhancing their passive diffusion. These studies highlight the diversity and heterogeneity in the hydrophobicity-induced nano-bio interactions at the cellular level and offer a new pathway to expediting cellular uptake of engineered nanoparticles. In addition, the amphiphilic luminescent AuNPs with high affinity to cell membrane and rapid endocytosis potentially serve as dual-modality imaging probes to correlate fluorescence and electron microscopies at the cellular level.