A polymorphic interstitial telomere array near the center of mouse chromosome 8.
A polymorphic interstitial telomere array near the center of mouse chromosome 8.
复制标题
小鼠 8 号染色体中心附近的多态性间质端粒阵列。
DOI：
10.1007/s003359900059
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Elliott,RW
中科院分区：
文献类型：
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作者：
Yen,CH;Pazik,J;Elliott,RW
两种间质阵列，Tel-rs1（端粒相关序列1）和tel -rs3，先前已在小鼠中报道过。本文报道了另一个短阵列的端粒六聚体（Tel-rs2）的鉴定。发现tel -rs2位于LINE 1序列的两侧，并插入LINE 1序列中，对LINE 1序列的干扰很小。这表明，这种间质阵列是染色体断裂部位的染色体愈合事件的结果。利用引物对不同小鼠品系的DNA序列flankingtel -rs2进行扩增，发现存在3个等位基因的多态性。通过对6个重组自交系tel -rs2等位基因的株系分布和不同鼠系回交中等位基因的分离分析表明，tel -rs2位于end8mit8和d8mit9之间的8号染色体上，与plandjund1有紧密的连锁关系。本文报道的核苷酸序列已提交至GenBank，登录号为U38220、U38221、U38222和U38223。
Two interstitial arrays,Tel-rs1(telomere-related sequence 1) andTel-rs3, have previously been reported in mice. This paper reports the identification of another short array of telomere hexamers (Tel-rs2).Tel-rs2was found to be flanked by sequences from a LINE 1 and was inserted into the LINE 1 sequence with very little perturbation of the LINE 1 sequence. It is suggested that this interstitial array is the result of a chromosomal healing event at the site of a chromosome break. Amplification of DNA from different mouse strains using primers based on the DNA sequence flankingTel-rs2indicated the presence of a polymorphism with 3 alleles. The strain distribution pattern ofTel-rs2alleles in 6 recombinant inbred strains and the segregation pattern of the alleles in backcrosses among different mouse strains indicated thatTel-rs2was located on chromosome 8 betweenD8Mit8andD8Mit9and that it was tightly linked toLplandJund1. The nucleotide sequences reported in this paper have been submitted to GenBank, accession numbers U38220, U38221, U38222 and U38223.