Expressed sequence tags reveal Proctotrupomorpha (minus Chalcidoidea) as sister to Aculeata (Hymenoptera: Insecta)
Expressed sequence tags reveal Proctotrupomorpha (minus Chalcidoidea) as sister to Aculeata (Hymenoptera: Insecta)
复制标题
DOI：
10.1016/j.ympev.2010.07.006
复制
发表时间：
2010-10-01
影响因子：
4.1
通讯作者：
Sharkey, Michael J.
中科院分区：
文献类型：
--
作者：
Sharanowski, Barbara J.;Robbertse, Barbara;Sharkey, Michael J.
膜翅目是地球上最多样化的动物类群之一，作为传粉者和寄生虫对生态系统功能至关重要。众所周知，用形态学和传统的分子方法很难解决Hyplutera之间的高层次关系。在这里，我们研究了实用程序的表达序列标签解决hyperteran超家族之间的关系。转录本组装为6个不同的Hyplitteran类群与额外的序列添加从公共数据库的最终数据集的24个基因的16个类群和超过10 kb的序列数据。连接的数据集恢复了一个强大的和良好的支持的拓扑结构，证明单系的Holometabola，Hyattera，Apocrita，Aculeata，Ichapronoidea，和姐妹关系之间的两个最密切相关的proctotrupomorphs的数据集（Cynipoidea + Proctotrupoidea）。这些数据强烈支持Aculeata和Proctotrupomorpha之间的姐妹关系，与以前提出的假设相反。此外，有强有力的证据表明，Ichalonoidea是Aculeata + Proctotrupomorpha的姐妹。这些关系对于缺失数据、核苷酸组成偏差、低分类学采样和跨基因树的冲突信号是稳健的。也有强有力的证据表明，Chalcidoidea不包含在Proctotrupomorpha。(C)2010年爱思唯尔公司All rights reserved.
Hymenoptera is one of the most diverse groups of animals on the planet and have vital importance for ecosystem function as pollinators and parasitoids. Higher-level relationships among Hymenoptera have been notoriously difficult to resolve with both morphological and traditional molecular approaches. Here we examined the utility of expressed sequence tags for resolving relationships among hymenopteran superfamilies. Transcripts were assembled for 6 disparate Hymenopteran taxa with additional sequences added from public databases for a final dataset of 24 genes for 16 taxa and over 10 kb of sequence data. The concatenated dataset recovered a robust and well-supported topology demonstrating the monophyly of Holometabola, Hymenoptera, Apocrita, Aculeata, Ichneumonoidea, and a sister relationship between the two most closely related proctotrupomorphs in the dataset (Cynipoidea + Proctotrupoidea). The data strongly supported a sister relationship between Aculeata and Proctotrupomorpha, contrary to previously proposed hypotheses. Additionally there was strong evidence indicating Ichneumonoidea as sister to Aculeata + Proctotrupomorpha. These relationships were robust to missing data, nucleotide composition biases, low taxonomic sampling, and conflicting signal across gene trees. There was also strong evidence indicating that Chalcidoidea is not contained within Proctotrupomorpha. (C) 2010 Elsevier Inc. All rights reserved.