Serum concentration of cartilage oligomeric matrix protein (COMP) is sensitive to physiological cyclic loading in healthy adults
Serum concentration of cartilage oligomeric matrix protein (COMP) is sensitive to physiological cyclic loading in healthy adults
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DOI：
10.1016/j.joca.2004.09.007
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发表时间：
2005-01-01
影响因子：
7
通讯作者：
King, KB
中科院分区：
文献类型：
--
作者：
Mündermann, A;Dyrby, CO;King, KB
目的：验证健康人群30min步行运动中生理循环负荷引起血清软骨低聚基质蛋白(COMP)浓度升高的假说。方法：10名体力活动成人分别于运动前、运动后即刻、30min步行后0.5h、1.5h、3.5h和5.5h采集血样(5ml)。在另一天，同样的10名受试者在6个月的时间里，当他们坐在椅子上时，采集了血液样本。采用商品化的酶联免疫吸附试验(CoMP(R)ELISA)测定血清CoMP浓度。活动监测器被用来记录步态的基本时间距离测量。结果：在运动方案中，步行运动后立即出现第一次升高(9.7%；P=0.003)。步行运动后5.5h，血清COMP浓度再次升高(7.0%；P=0.024)。在静息方案中，基线浓度显著高于随后的所有时间点(8.2%；P<；0.050)。血清CoMP浓度从3.5h下降到5.5h(-4.8%；P=0.012)。结论：即使是中等程度的步行活动也会显著影响血清CoMP浓度。直接的反应是COMP碎片从软骨扩散到血液的时间不超过30分钟。5.5小时的反应表明COMP的代谢延迟在5小时到6小时的范围内。(C)2004年国际骨性关节炎研究学会。爱思唯尔有限公司出版。保留所有权利。
Objective: To test the hypothesis that physiological cyclic loading during a 30-min walking exercise causes an increase in serum cartilage oligomeric matrix protein (COMP) concentration in a healthy population.Methods: Blood samples (5 ml) were drawn from 10 physically active adults immediately before and after, and 0.5 h, 1.5 h, 3.5 h and 5.5 h after a 30-min walking exercise on a level outdoor walking track at self-selected normal speed. On a separate day, blood samples were drawn from the same 10 subjects during 6 In while they were resting in a chair. Serum COMP concentrations were determined using a commercial enzyme-linked immunosorbent assay (COMP(R) ELISA). An activity monitor was used to record basic time-distance measurements of gait. Serum COMP concentrations within the exercise protocol and within the resting protocol were compared using separate repeated measures analyses of variance (alpha = 0.05).Results: In the exercise protocol, a first increase (9.7%; P = 0.003) occurred immediately after the walking exercise. A second increase in serum COMP concentration (7.0%; P = 0.024) occurred 5.5 h after the walking exercise. In the resting protocol, the concentration at baseline was significantly higher than at all subsequent time points (8.2%; P < 0.050). Serum COMP concentration decreased from the 3.5-h to the 5.5-h sample (-4.8%; P = 0.012).Conclusions: Even a moderate walking activity can significantly influence serum COMP concentration. The immediate response points to a diffusion time of COMP fragments from cartilage to the blood of 30 min or less. The response at 5.5 h indicates a metabolic delay for COMP in the range of 5 h to 6 h. (C) 2004 OsteoArthritis Research Society International. Published by Elsevier Ltd. All rights reserved.