Cholesterol side-chain cleavage activity in the adrenal gland of the young rat: development and responsiveness to adrenocorticotropin hormone.
Cholesterol side-chain cleavage activity in the adrenal gland of the young rat: development and responsiveness to adrenocorticotropin hormone.
复制标题
幼鼠肾上腺中的胆固醇侧链裂解活性：发育和对促肾上腺皮质激素的反应。
DOI：
10.1210/endo-85-3-500
复制
发表时间：
1969
期刊：
影响因子：
4.8
通讯作者：
R. B. Clayton
中科院分区：
文献类型：
--
作者：
C. Doering;R. B. Clayton
描述了一种改进的方法，用于测定大鼠肾上腺中胆固醇侧链裂解的酶活性。该方法利用双同位素标记，可以方便地应用于比迄今为止更小的组织样本。利用这种改进的技术，对 1 至 46 天不同年龄的大鼠肾上腺的胆固醇侧链裂解活性进行了研究，并报告了结果。 1日龄大鼠肾上腺酶活性相对较高，但在出生后前6天下降，直到出生后第12天左右保持较低水平，然后在第25天左右上升到较高水平。这种发育模式与已报道的新生大鼠肾上腺对应激的反应密切相关。连续8天重复注射ACTH（促肾上腺皮质激素）可以防止新生儿肾上腺裂解活性的下降。 ACTH 治疗 2 天效果并不理想...
An improved method is described for the assay of the enzymic activity of cholesterol side-chain cleavage in rat adrenal glands. The method makes use of double isotope labeling and can be conveniently applied to smaller tissue samples than has been possible hitherto. With this improved technique, a study of the cholesterol side-chain cleavage activity of the adrenals of rats of various ages, from 1 to 46 days, has been undertaken and the results are reported. The enzyme activity is relatively high in the adrenal gland of the 1-day-old rat, but falls during the first 6 postnatal days, remains low until about the 12th postnatal day, then rises to a higher level by about the 25th day. This developmental pattern correlates closely with that which has been reported for the responsiveness of the adrenal of the neonatal rat to stress. Repeated injections of ACTH (adrenocorticotropic hormone) for 8 days prevent the decline of cleavage activity in the adrenal of the newborn. Treatment with ACTH for 2 days is not lo...