A novel electrochemical biosensor for sensitive detection of telomerase activity based on structure-switching DNA.
A novel electrochemical biosensor for sensitive detection of telomerase activity based on structure-switching DNA.
复制标题
DOI：
10.1016/j.bios.2013.09.072
复制
发表时间：
2014-03
影响因子：
12.6
通讯作者：
Zi Yi;Hai-bo Wang;Ke Chen;Qing Gao;Hao Tang;R. Yu;X. Chu
中科院分区：
文献类型：
--
作者：
Zi Yi;Hai-bo Wang;Ke Chen;Qing Gao;Hao Tang;R. Yu;X. Chu
端粒酶被认为是一种重要的肿瘤生物标志物，可用于肿瘤的早期诊断和治疗。以二茂铁（Fc）为电活性报告分子，构建了一种基于结构转换DNA探针的端粒酶电化学生物传感器。该方法的动态范围为102 ~ 6× 104 Hela细胞mL-1，检测限为100 Hela细胞mL-1。该生物传感器具有良好的重现性、稳定性和操作简单等优点。该方法为端粒酶活性定量检测的临床应用提供了一个实用的平台。端粒酶抑制剂的筛选结果表明，该方法适用于端粒酶抑制剂的筛选研究。
Telomerase has been considered to be an important tumor biomarker for early cancer diagnostics and a valuable target for therapy treatment. A novel electrochemical biosensor based on structure-switching DNA probe with ferrocene (Fc) as the electroactive reporter to detect telomerase activity was developed. The developed approach displayed desirable dynamic range from 102to 6×104Hela cells mL−1with a detection limit of 100 Hela cells mL−1. This biosensor afforded good reproducibility, stability and simple operations. It provided a useful platform for practical use in quantitative telomerase activity assay for clinical applications. Telomerase inhibitor performance was also investigated and the results indicated the approach was suitable for telomerase inhibitor screening research.