New lineage of filamentous, spore-forming, gram-positive bacteria from soil
New lineage of filamentous, spore-forming, gram-positive bacteria from soil
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DOI：
10.1128/aem.00132-06
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发表时间：
2006-06-01
影响因子：
4.4
通讯作者：
Donadio, Stefano
中科院分区：
文献类型：
--
作者：
Cavaletti, Linda;Monciardini, Paolo;Donadio, Stefano
从意大利土壤中分离的命名为SOSP 1 -21（T）的新细菌菌株在固体和液体培养基中形成分支菌丝体，并具有与属于放线菌的某些属相似的丝状形态。电子显微镜显示，这种生物体具有葡萄状外观，这是由产孢菌丝产生的孢子交织而成的。从土壤中分离到10株与S 0 SP1 -21（T）形态学相关的菌株。16 S rRNA基因片段的系统发育分析证实了这些菌株与SOSP 1 -21（T）的相关性，并表明新分离的菌株在一个深分支谱系中形成单独的分支。所有菌株的16 S rRNA序列的最接近匹配（约79%的同一性）与Chloroflexi的代表相匹配，尽管与该部门的联系没有得到高自举值的支持。这些菌株是嗜温需氧异养菌，也能够在微需氧条件下生长。它们都是革兰氏阳性。菌株SOSP 1 -21（T）含有鸟氨酸、丙氨酸、谷氨酸、丝氨酸和甘氨酸作为肽聚糖氨基酸。此外，一个不寻常的水平C16：1 20 H（30%）被发现在细胞脂肪酸。SOSPI-21（T）基因组DNA的G+C含量为53.9%，MK-9（H-2）是唯一检测到的甲基萘醌。所有这些数据表明，SOSP 1 -21（T）和相关菌株可能构成一个新的分支的丝状，孢子形成，革兰氏阳性细菌。我们建议命名为Redobacter racentifer gen. nov.，sp. nov.）的菌株SOSP 1 -21（T）。
A novel bacterial strain that was isolated from an Italian soil and was designated SOSP1-21(T) forms branched mycelia in solid and liquid media and has a filamentous morphology similar to that of some genera belonging to the Actinobacteria. Electron microscopy showed that this organism has a grape-like appearance, resulting from interlacing of spores originating from sporophoric hyphae. Ten strains that are morphologically related to S0SP1-21(T) were recovered from soil. Phylogenetic analyses of 16S rRNA gene segments confirmed the relatedness of these strains to SOSP1-21(T) and indicated that the newly isolated strains form separate clades in a deeply branching lineage. The closest matches for the 16S rRNA sequences of all the strains (around 79% identity) were matches with representatives of the Chloroflexi, although the affiliation with this division was not supported by high bootstrap values. The strains are mesophilic aerobic heterotrophs and are also capable of growing under microaerophilic conditions. They all stain gram positive. Strain SOSP1-21(T) contains ornithine, alanine, glutamic acid, serine, and glycine as the peptidoglycan amino acids. In addition, an unusual level of C16:1 20H (30%) was found in the cellular fatty acids. The G+C content of SOSPI-21(T) genomic DNA is 53.9%, and MK-9(H-2) was the only menaquinone detected. All these data suggest that SOSP1-21(T) and the related strains may constitute a new division of filamentous, spore-forming, gram-positive bacteria. We propose the name Redobacter racentifer gen. nov., sp. nov. for strain SOSP1-21(T).