Development of an indirect ELISA method for the parallel measurement of IgG and IgM antibodies against Crimean-Congo haemorrhagic fever (CCHF) virus using recombinant nucleoprotein as antigen
Development of an indirect ELISA method for the parallel measurement of IgG and IgM antibodies against Crimean-Congo haemorrhagic fever (CCHF) virus using recombinant nucleoprotein as antigen
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DOI：
10.1016/j.jviromet.2011.11.020
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发表时间：
2012-02-01
影响因子：
3.1
通讯作者：
Hewson, R.
中科院分区：
文献类型：
--
作者：
Dowall, S. D.;Richards, K. S.;Hewson, R.
从克里米亚-刚果出血热（CCHF）病毒的重组核蛋白成功地从杆状病毒表达系统中获得，并纯化用于新的酶联免疫吸附试验（ELISA）诊断试验。使用可比试验检测IgG和IgM抗体。从而允许平行地有效检测两种抗体。该检测方法的主要优点还包括消除了对多克隆血清的任何要求，从而消除了制备过程中的差异，并允许实验室之间的标准化。使用从土耳其、塔吉克斯坦和科索沃鉴定为阳性的样品中提供的一组阳性血清成功地测试了该测定法，并显示其灵敏度和特异性。据设想，这种用于CCHF病毒感染的简单诊断ELISA消除了对多克隆抗体制剂的依赖，将可用于更广泛的实验室，使其能够进行常规诊断。这将提高诊断和随后管理受感染患者的效率。皇冠版权所有（C）2011由Elsevier B. V.出版。保留所有权利。
Recombinant nucleoprotein from Crimean-Congo Haemorrhagic Fever (CCHF) virus was successfully derived from a baculovirus expression system and purified for use in a novel enzyme-linked immunosorbent assay (ELISA) diagnostic test. Comparable tests were used for detection of IgG and IgM antibodies. thus allowing efficient detection of both antibodies in parallel. The major benefits of the assay also included removing any requirement for polyclonal sera, thus eliminating variation in preparations and allowing standardisation between laboratories. The assay was successfully tested using a panel of positive sera supplied from samples identified as being positive in Turkey, Tajikistan and Kosovo and shown to be sensitive and specific. It is envisaged that this simple diagnostic ELISA for CCHF virus infection which removes the reliance on polyclonal antibody preparations, will be accessible to a wider range of laboratories enabling them to carry out routine diagnosis. This will improve the efficiency of diagnosis and subsequent management of infected patients. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.