YjgF is required for isoleucine biosynthesis when Salmonella enterica is grown on pyruvate medium
YjgF is required for isoleucine biosynthesis when Salmonella enterica is grown on pyruvate medium
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DOI：
10.1128/jb.01700-07
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发表时间：
2008-04-01
影响因子：
3.2
通讯作者：
Downs, Diana M.
中科院分区：
文献类型：
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作者：
Christopherson, Melissa R.;Schmitz, G. E.;Downs, Diana M.
YJGF/YER057C/UK114蛋白质家族在生命的三个领域中保存下来，但是对于任何家族的任何成员来说，都没有明确定义生化功能。在肠道沙门氏菌中，当在葡萄糖丝氨酸或丙酮酸培养基中生长突变菌株时，YJGF的缺失会导致异亮氨酸。异亮氨酸对葡萄糖丝氨酸培养基的治愈作用需要抑制ILVA的反馈抑制。在丙酮酸培养基上，YJGF突变体无法合成足够的异亮氨酸以生长。从这项研究中，我们得出结论，丙酮酸YJGF突变体的异亮氨酸需求是跨激酶B（ILVE）活性降低的结果，该活性先前在这些突变体中已经表征了。
The YjgF/YER057c/UK114 family of proteins is conserved across the three domains of life, yet no biochemical function has been clearly defined for any member of this family. In Salmonella enterica, a deletion of yjgF results in a requirement for isoleucine when the mutant strain is grown in glucose-serine or pyruvate medium. Feedback inhibition of IlvA is required for the curative effect of isoleucine on glucose-serine medium. On pyruvate medium, yjgF mutants are unable to synthesize enough isoleucine for growth. From this study, we conclude that the isoleucine requirement of a yjgF mutant on pyruvate is a consequence of the decreased transaminase B (IlvE) activity that has previously been characterized in these mutants.