Reorganization of primary afferent nerve terminals in the spinal dorsal horn of the primate caudal to anterolateral chordotomy.
Reorganization of primary afferent nerve terminals in the spinal dorsal horn of the primate caudal to anterolateral chordotomy.
复制标题
灵长类动物脊索背角初级传入神经末梢的重组，从尾部到前外侧脊索切开术。
DOI：
10.1002/cne.902700408
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Perl,ER
中科院分区：
文献类型：
--
作者：
Bullitt,E;Stofer,WD;Vierck,CJ;Perl,ER
一种灵长类动物模型已经被用来探索前外侧脊索切断术可能导致初级传入神经终末在椎管内发芽或重新排列，这可能是术后感觉功能恢复延迟的原因。猴子被训练来限制电刺激的持续时间，并用它们逃避反应的强度和频率来区分痛苦和非痛苦的刺激水平。行为测试显示：(1)所有动物均出现对侧痛觉减退，有一半的动物感觉恢复；(2)所有动物的双侧反射力降低，大部分猴的反射恢复。在终末实验中，用HRP标记脊髓损伤尾侧的背根，并测定标记的突触复合体在背角内的分布。与对照组相比，接受脊索切断术的动物表现出浅层背角终末的丢失和深层突触增大的增加。这些影响是双侧的，但在脉络膜切断术的对侧最为明显。弥漫性脊髓损伤的动物在初级传入终末的分布上显示出完全不同的变化。与持续痛觉减退的猴子相比，感觉恢复的动物表现出更正常的终末分布模式，但数据存在相当大的变异性，不同统计检验的分析得出了不同的结果。
A primate model has been used to explore the possibility that anterolateral chordotomy may produce intraspinal sprouting or rearrangement of primary afferent nerve terminations that could account for delayed postoperative recovery of sensory function. Monkeys were trained to limit the duration of an electrical stimulus, and the vigor and frequency of their escape responses were used to differentiate painful from nonpainful levels of stimulation. Behavioral testing after chordotomy revealed: (1) contralateral hypalgesia in all animals, with sensory recovery in half of the group, and (2) bilateral decreases in reflexive force in all animals, with reflex recovery in the majority of monkeys. At the terminal experiment, dorsal rootlets caudal to the spinal lesion were labeled bilaterally with HRP, and the distribution of labeled synaptic complexes was determined within the dorsal horn. When compared to controls, animals undergoing chordotomy showed a loss of terminals in the superficial dorsal horn and an increase of synaptic enlargements in deeper layers. These effects were bilateral, but were most pronounced on the side contralateral to chordotomy. Animals with diffuse spinal lesions showed a completely different change in the distribution of primary afferent terminals. Animals with sensory recovery demonstrated a more normal terminal distribution pattern than persistently hypalgesic monkeys, but there was considerable variability in the data, and analysis by different statistical tests yielded varying results.