UNSTABLE RIBONUCLEIC ACID REVEALED BY PULSE LABELLING OF ESCHERICHIA COLI

UNSTABLE RIBONUCLEIC ACID REVEALED BY PULSE LABELLING OF ESCHERICHIA COLI
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DOI:
10.1038/190581a0
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发表时间:
1961-01-01
期刊:
影响因子:
64.8
通讯作者:
RISEBROUGH, RW
RISEBROUGH, RW
中科院分区:
综合性期刊1区
文献类型:
--
作者:
GROS, F;KURLAND, CG;RISEBROUGH, RW

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通过暴露大肠杆菌短脉冲(10-20秒)通过对放射性RNA前体进行分析,并通过蔗糖梯度离心法检查合成的RNA,可以识别出与核糖体或转移RNA不同的不稳定的RNA组分。这种不稳定组分在沉降常数和与T2特异性RNA的附着性质上相似,被认为是信使RNA[cf.4211]。
By exposingEscherichia colito short pulses (10-20 sec.) of radioactive RNA precursors and examining the synthesized RNA by sucrose-gradient centrifugation, it has been possible to identify an unstable RNA fraction, distinct from ribosomal or transfer RNA. This unstable fraction is similar in sedimentation constant and attachment properties to T2-specific RNA and is thought to be messenger RNA [cf. 4211].