SEX-DIFFERENCES IN THE DENSITIES OF EPIDERMAL LANGERHANS CELLS OF THE MOUSE
SEX-DIFFERENCES IN THE DENSITIES OF EPIDERMAL LANGERHANS CELLS OF THE MOUSE
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DOI：
10.1111/1523-1747.ep12470104
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发表时间：
1987-05-01
影响因子：
6.5
通讯作者：
MARUNOUCHI, T
中科院分区：
文献类型：
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作者：
KOYAMA, Y;NAGAO, S;MARUNOUCHI, T
已知皮肤免疫反应表现出性别二态性。朗格汉斯细胞（LCs）是表皮中的骨髓源性免疫细胞，对皮肤的免疫反应至关重要。本研究对雌雄小鼠LC密度的差异进行了研究。采用乙二胺四乙酸（EDTA）处理方法，从SPF小鼠后肢和耳朵无毛部位皮肤上分离表皮片，进行腺苷三磷酸酶（ATPase）活性染色。雄性C57BL/6后肢表皮上皮细胞密度(823 .+-。20/mm2)和BALB/c(1689 .+-。66/mm2)，显著低于雌性(1363 +-。52/mm2, p < 0.001；2249 + -。105/mm2, p < 0.001)。雄性C57BL/6耳朗格汉斯细胞密度(465 +-。24/mm2)，也显著低于雌性（542 +-）。17/mm2, p < 0.02)。虽然卵巢切除对雌性C57BL/6小鼠后肢表皮LC密度没有任何影响，但雄性C57BL/6小鼠的LC密度在睾丸切除后4周（假手术，564 .+-）显著增加。27 /平方毫米;阉割，1179。49/mm2, p < 0.001)。这些结果表明，小鼠表皮LC密度与性别有关，即雄性小鼠的LC比雌性小鼠少。男性LC密度降低可能与睾丸有关。
Cutaneous immune reactions are known to show sexual dimorphism. Langerhans cells (LCs) are bone marrow-derived immune cells in the epidermis and are essential to immune reactions in the skin. In the present research, a study was made of the differences in LC density of male and female mice. Epidermal sheets were separated from the skin of the glabrous part of hind limbs and ears of specific pathogen-free (SPF) mice by ethylenediaminetetraacetic acid (EDTA) treatment and stained for adenosine triphosphatase (ATPase) activity. The density of LCs of hind limb epidermis in male C57BL/6 (823 .+-. 20/mm2) and BALB/c (1689 .+-. 66/mm2) mice was significantly less than that in females (1363 .+-. 52/mm2, p < 0.001; 2249 .+-. 105/mm2, p < 0.001, respectively). Langerhans cell density in the ears of male C57BL/6 (465 .+-. 24/mm2) mice was also significantly less than that in females (542 .+-. 17/mm2, p < 0.02). Although ovariectomy failed to bring about any change in the LC density of hind limb epidermis in female C57BL/6 mice, the LC density in male C57BL/6 mice increased significantly at 4 weeks following orchiectomy (sham operation, 564 .+-. 27/mm2; castration, 1179 .+-. 49/mm2, p < 0.001). These results indicate that mouse epidermal LC density depends on sex, i.e., male mice have fewer LCs than female mice. The reduction in LC density in males may possibly be caused by the testis.