Mussel-Inspired Peptide Coatings on Titanium Implant to Improve Osseointegration in Osteoporotic Condition
Mussel-Inspired Peptide Coatings on Titanium Implant to Improve Osseointegration in Osteoporotic Condition
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钛植入物上受贻贝启发的肽涂层可改善骨质疏松症的骨整合
DOI：
10.1021/acsbiomaterials.8b00261
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发表时间：
2018
影响因子：
--
通讯作者：
Shi Qin
中科院分区：
文献类型：
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作者：
Zhao Huan;Huang Yingkang;Zhang Wen;Guo Qianping;Cui Wenguo;Sun Zhiyong;Eglin David;Liu Lei;Pan Guoqing;Shi Qin
由于骨质疏松症条件下骨生成不良和植入物（如钛基生物材料）的生物活性低，骨质疏松症极大地损害了体内植入物的骨整合。已经开发了各种表面工程策略，包括不稳定的物理吸收或复杂的化学缀合，以使钛植入物生物功能化并改善界面骨整合。然而，很少有人考虑到临床上具有挑战性的骨质疏松症，以及植入物的双功能化，以改善成骨细胞粘附和成骨。在这项工作中，我们结合了两种贻贝启发的生物活性肽（即，具有细胞粘附或成骨序列），通过简单的自组织多价协调相互作用对钛螺钉进行一步双功能化。体外研究表明，仿生双功能涂层能有效促进骨代谢受损的骨髓间充质干细胞的成骨作用。此外，在体内条件下，钛螺钉上的双功能肽涂层也可以显著增强界面成骨、新骨形成条件、骨整合以及种植体机械稳定性。这可能是由于改良钛螺钉上的整合素靶向细胞粘附和成骨基序，在骨质疏松条件下恢复了骨生成和破骨细胞生成之间的正常骨代谢平衡。我们预计，高度仿生肽和一步双功能化的策略将提供一个简单而有效的手段，改善钛基植入物在骨代谢紊乱的患者的临床结果。
Osteoporosis greatly impairs in vivo implant osseointegration because of poor osteogenesis in osteoporotic conditions and the low bioactivity of implants, such as titanium-based biomaterials. Various surface engineering strategies, including unstable physical absorption or complex chemical conjugations, have been developed to biofunctionalize titanium implants and improve interfacial osseointegration. However, very few of them took into consideration the clinically challenging osteoporotic condition, as well as dual-functionalization of the implants for improvement of both osteoblast adhesion and osteogenesis. In this work, we combined two mussel-inspired bioactive peptides (i.e., with cell adhesive or osteogenic sequences) for one-step dual-functionalization of Ti screws via a facile self-organized multivalent coordinative interaction. In vitro study indicated that the biomimetic dual-functional coating could efficiently improve the osteogenesis of osteoporosis-derived mesenchymal stem cells despite of their impaired bone metabolism. Moreover, under osteoporotic in vivo condition, the dual-functional peptide coating on Ti screws could also give rise to significant enhancement of interfacial osteogenesis, newly formed bone condition, osseointegration, as well as implant mechanical stability. This is probably due to the integrin-targeted cell adhesive and osteogenic motifs on the modified Ti screws, which recovered the regular bone metabolism equilibrium between osteogenesis and osteoclastogenesis in an osteoporotic condition. We anticipate that the highly biomimetic peptides and one-step dual-functionalized strategy would provide a facile and effective means for improving the clinical outcome of Ti-based implants in patients with a disturbed bone metabolism.