Status of cathelicidin IL-37, cytokine TNF, and vitamin D in patients with pulmonary tuberculosis.
Status of cathelicidin IL-37, cytokine TNF, and vitamin D in patients with pulmonary tuberculosis.
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发表时间：
2018-03
影响因子：
3.2
通讯作者：
K. Majewski;J. Agier;E. Kozłowska;E. Brzezińska‐Błaszczyk
中科院分区：
文献类型：
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作者：
K. Majewski;J. Agier;E. Kozłowska;E. Brzezińska‐Błaszczyk
结核分枝杆菌（Mtb）感染的发展取决于宿主引发对病原体的保护性免疫应答的能力。Cathelicidin在抗菌天然免疫机制中发挥作用。这种肽有助于呼吸道上皮的屏障功能，并参与控制肺部细菌感染。LL-37（leucine-leucine-37）也参与对分枝杆菌感染的宿主防御和先天免疫应答。本研究旨在评估活动性肺结核（TB）患者血清LL-37浓度，并确定LL-37肽、肿瘤坏死因子（TNF）和维生素D血清水平之间是否存在任何相关性。总共招募了46名患有肺结核的成年人参加这项研究。随机抽取61名健康对照者作为对照组。使用酶联免疫吸附测定（ELISA）试剂盒测定cathelicidin LL-37、维生素D（25（OH）D）以及TNF的血清浓度。与健康对照组（1.41±0.22）相比，TB组（7.45±1.58）中LL-37的平均（± SEM）水平显著更高（p <0.001）。TB组的平均血清TNF浓度（8.51±1.92）显著高于健康对照组（2.69±0.19）（p <0.001）。健康人血清维生素D水平（26.10±1.74）与结核病患者血清维生素D水平（24.18±1.95）无显著性差异。未观察到TB患者LL-37、TNF和维生素D水平之间的相关性。我们的研究结果表明，血清中的肽LL-37的水平在结核病显着提高，这一观察是兼容的一般观点的重要作用，这种cathelicidin防御机制对结核分枝杆菌感染。
Development of Mycobacterium tuberculosis (Mtb) infection depends on the ability of the host to elicit the protective immune response to the pathogen. Cathelicidin plays a role in antibacterial innate immunity mechanisms. This peptide contributes to the barrier function of respiratory epithelium and takes part in controlling pulmonary bacterial infections. LL-37 (leucine-leucine-37) is involved in host defense and innate immune response to mycobacterial infections, as well. This study aims to evaluate the serum concentrations of LL-37 in individuals with active pulmonary tuberculosis (TB) and to determine whether any correlations between peptide LL-37, tumor necrosis factor (TNF) and vitamin D serum levels exist. A total of 46 adults with pulmonary TB were recruited for the study. Sixty-one controls were randomly selected as control group. Serum concentrations of cathelicidin LL-37, vitamin D (25(OH)D), as well as TNF, were measured using an enzyme-linked immunosorbent assay (ELISA) kit. The mean (± SEM) level of LL-37 was significantly higher in the TB group (7.45±1.58) compared with healthy controls (1.41±0.22) (p less than 0.001). Mean serum concentration of TNF was significantly higher in the TB group (8.51±1.92) compared with healthy controls (2.69±0.19) (p less than 0.001). There was no significant difference in mean serum levels of vitamin D between healthy (26.10±1.74) and TB subjects (24.18±1.95). No correlations between LL-37, TNF, and vitamin D levels in patients with TB were observed. Our results indicated that serum levels of peptide LL-37 during TB is raised significantly, and this observation is compatible with the general view of the important role of this cathelicidin in defense mechanisms against Mtb infection.