Low salinity affects cellularity, DNA methylation, and mRNA expression of igf1 in the liver of half smooth tongue sole (Cynoglossus semilaevis)
Low salinity affects cellularity, DNA methylation, and mRNA expression of igf1 in the liver of half smooth tongue sole (Cynoglossus semilaevis)
复制标题
低盐度影响半滑舌鳎 (Cynoglossus semilaevis) 肝脏中的细胞结构、DNA 甲基化和 igf1 mRNA 表达
DOI：
10.1007/s10695-017-0395-7
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发表时间：
2017-12-01
影响因子：
2.9
通讯作者：
Meng, Lingcai
中科院分区：
文献类型：
--
作者：
Li, Siping;He, Feng;Meng, Lingcai
动物的生长取决于激素水平和环境条件的反馈调节。胰岛素样生长因子-1（Igf 1）促进细胞生长和分化，抑制细胞凋亡，并受环境高度调节。此外，动物通过表观遗传学和遗传调节机制在应激条件下改变生理稳态。因此，需要全面了解盐对鱼类生长的影响。以半滑舌鳎（Cynoglossus semilaevis）为材料，研究了低盐度对半滑舌鳎肝脏细胞结构和生长的影响。结果表明，低盐度改变了肝脏的形态，这表明从渗透破坏中恢复的能量消耗增加。15‰盐度下雌鱼7 D后igf 1表达上调，可能参与分子修复。盐胁迫60 d后igf 1表达下调，生长受阻。甲基化水平与基因表达水平相反，表明调控受到抑制。此外，igf 1基因的三个外显子在盐胁迫下有显著不同的甲基化水平。值得注意的是，更多的假定的转录因子结合位点位于CpG位点在较高的甲基化水平。igf 1不是一个性别相关基因，因为在对照组的男性和女性之间没有检测到甲基化水平的差异。这些结果阐明了肝脏损伤和DNA甲基化以及igf 1 mRNA表达的变化，为了解低盐对C. semilaevis和表观遗传学和调节机制参与应激条件。
Animal growth depends on feedback regulation of hormone levels and environmental conditions. Insulin-like growth factor-1 (Igf1) promotes cell growth and differentiation and represses apoptosis and is highly regulated by the environment. Moreover, animals modify physiological homeostasis under stressful conditions through epigenetics and genetic regulatory mechanisms. Therefore, a comprehensive understanding of the effects of salt on fish growth is needed. In this study, half smooth tongue sole (Cynoglossus semilaevis) were subjected to 15‰ salinity for 0, 7, and 60 days (D) to assess the effects of low salinity on liver cellularity and growth. The results show that low salinity changed liver morphology, suggesting an increase in energy expenditure to recover from the osmotic disruption. igf1 was upregulated in female fish under 15‰ salinity after 7D and may participate in molecular repair. igf1 was downregulated after 60D of salt stress, resulting in retarded growth. Methylation levels were opposite to those of gene expression, suggesting inhibited regulation. Furthermore, three exons in the igf1 gene had significantly different methylation levels in fish under salt stress. Notably, more putative transcription factor binding sites were located in CpG sites at higher methylation levels. igf1 is not a sex-related gene, as no difference in methylation level was detected between males and females in the control group. These results clarify liver damage and changes in DNA methylation and mRNA expression of igf1, providing insight into the adverse effects of low salt on growth of C. semilaevis and the epigenetics and regulatory mechanisms involved in stressful conditions.