Cyclic Stretch and Perfusion Bioreactor for Conditioning Large Diameter Engineered Tissue Tubes.
Cyclic Stretch and Perfusion Bioreactor for Conditioning Large Diameter Engineered Tissue Tubes.
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DOI：
10.1007/s10439-015-1437-x
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发表时间：
2016-05
影响因子：
3.8
通讯作者：
Tranquillo RT
中科院分区：
文献类型：
--
作者：
Schmidt JB;Tranquillo RT
设计了一种循环拉伸和灌注生物反应器来培养用于心脏瓣膜应用的大直径工程组织管。在该生物反应器中，将由牺牲性纤维蛋白凝胶支架中的真皮成纤维细胞组成的管状组织放置在穿孔的乳胶支撑套筒上，并安装在定制的生物反应器中。培养介质脉动流入系统导致循环拉伸以及外腔、内腔和透壁流动（灌注）。在本研究中，比较了在该生物反应器中循环拉伸的组织的管腔重塑、组成、机械强度和刚度，这些组织位于多孔乳胶袖套或固体乳胶袖套上，不允许管腔或透壁流动。在有孔袖套上周期性拉伸的组织具有局部于乳胶袖套中的孔柱的管腔重塑和细胞结构增加的区域。使用COMSOL Multiphysics®开发了CFD模型，以预测组织内和周围的培养基流动，预测结果表明，增强的管腔重塑可能是这些区域剪切应力和透壁速度升高的结果。这项工作突出了增加营养转运和流动刺激对加速体外组织重塑的有益作用。
A cyclic stretch and perfusion bioreactor was designed to culture large diameter engineered tissue tubes for heart valve applications. In this bioreactor, tubular tissues consisting of dermal fibroblasts in a sacrificial fibrin gel scaffold were placed over porated latex support sleeves and mounted in a custom bioreactor. Pulsatile flow of culture medium into the system resulted in cyclic stretching as well as ablumenal, lumenal, and transmural flow (perfusion). In this study, lumenal remodeling, composition, and mechanical strength and stiffness were compared for tissues cyclically stretched in this bioreactor on either the porated latex sleeves or solid latex sleeves, which did not permit lumenal or transmural flow. Tissues cyclically stretched on porated sleeves had regions of increased lumenal remodeling and cellularity that were localized to the columns of pores in the latex sleeve. A CFD model was developed with COMSOL Multiphysics® to predict flow of culture medium in and around the tissue, and the predictions suggest that the enhanced lumenal remodeling was likely a result of elevated shear stresses and transmural velocity in these regions. This work highlights the beneficial effects of increased nutrient transport and flow stimulation for accelerating in vitro tissue remodeling.