INCOMPLETE ENTRY OF BACTERIOPHAGE-T7 DNA INTO F-PLASMID-CONTAINING ESCHERICHIA-COLI
INCOMPLETE ENTRY OF BACTERIOPHAGE-T7 DNA INTO F-PLASMID-CONTAINING ESCHERICHIA-COLI
复制标题
DOI：
10.1128/jb.177.14.4077-4083.1995
复制
发表时间：
1995-07-01
影响因子：
3.2
通讯作者：
MOLINEUX, IJ
中科院分区：
文献类型：
--
作者：
GARCIA, LR;MOLINEUX, IJ
通过测量进入基因组的 Dam 甲基化来确定噬菌体 T7 DNA 渗透到含有 F 质粒的大肠杆菌细胞中。无法有效感染含 F 细胞的 T7 菌株无法在感染中止之前将其 DNA 完全转移到细胞中。基因组前 44% 的输入正常发生在含有 F 的细胞中，但其余部分的输入是异常的。通过将裸DNA转染到感受态细胞中来绕过T7基因组进入的正常模式无法抑制F对噬菌体发育的排除。然而，来自高拷贝数质粒的各种无毒 T7 1.2 等位基因的过表达或来自 T7 基因组的 T3 1.2 的表达允许噬菌体在 F.
The penetration of bacteriophage T7 DNA into F plasmid-containing Escherichia coli cells was determined by measuring Dam methylation of the entering genome. T7 strains that cannot productively infect F-containing cells fail to completely translocate their DNA into the cell before the infection aborts. The entry of the first 44% of the genome occurs normally in an F containing cell, but the entry of the remainder is aberrant. Bypassing the normal mode of entry of the T7 genome by transfecting naked DNA into competent cells fails to suppress F exclusion of phage development. However, overexpression of various nontoxic T7 1.2 alleles from a high-copy-number plasmid or expression of T3 1.2 from a T7 genome allows phage growth in the presence of F.