Microfluidic Devices for Behavioral Analysis, Microscopy, and Neuronal Imaging in Caenorhabditis elegans
Microfluidic Devices for Behavioral Analysis, Microscopy, and Neuronal Imaging in Caenorhabditis elegans
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DOI：
10.1007/978-1-4939-2842-2_12
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发表时间：
2015-01-01
期刊：
影响因子：
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通讯作者：
Albrecht, Dirk R.
中科院分区：
文献类型：
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作者：
Lagoy, Ross C.;Albrecht, Dirk R.
微流控设备为线虫的研究提供了几个优势，特别是在呈现精确的物理和化学环境、在成像过程中固定动物、量化行为和自动化筛选方面。然而，在该领域广泛采用它们的挑战包括：比传统方法增加的复杂性、操作问题(如堵塞、泄漏和气泡)、获得或制造设备的困难，以及需要表征从新的检测格式获得的生物学结果。在这里，我们描述了用于量化对化学模式的行为反应的简单、可重复使用的微流控设备的制备和操作，以及用于安排动物进行时间推移显微镜和测量神经元活动的一次性设备。我们专注于消除或减少微流控设备新用户通常经历的挫折的细节。
Microfluidic devices offer several advantages for C. elegans research, particularly for presenting precise physical and chemical environments, immobilizing animals during imaging, quantifying behavior, and automating screens. However, challenges to their widespread adoption in the field include increased complexity over conventional methods, operational problems (such as clogging, leaks, and bubbles), difficulty in obtaining or fabricating devices, and the need to characterize biological results obtained from new assay formats. Here we describe the preparation and operation of simple, reusable microfluidic devices for quantifying behavioral responses to chemical patterns, and single-use devices to arrange animals for time-lapse microscopy and to measure neuronal activity. We focus on details that eliminate or reduce the frustrations commonly experienced by new users of microfluidic devices.