Chordin is required for the Spemann organizer transplantation phenomenon in Xenopus embryos
Chordin is required for the Spemann organizer transplantation phenomenon in Xenopus embryos
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DOI：
10.1016/s1534-5807(02)00404-5
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发表时间：
2003-02-01
影响因子：
11.8
通讯作者：
De Robertis, EM
中科院分区：
文献类型：
--
作者：
Oelgeschläger, M;Kuroda, H;De Robertis, EM
分析了非洲爪蟾发育过程中对Chordin的需求。用吗啉代反义寡聚体（Chd-MO）靶向爪蟾的两个脊索蛋白假等位基因显着降低了脊索蛋白的产生。胚胎发育与适度减少dorsoanterior结构和扩大ventroposterior组织，phenocopying斑马鱼chordino突变。一个强烈的要求脊索蛋白在背部发育的实验操作显示。首先，氯化锂处理的背侧化被Chd-MO完全阻断。其次，Chd-MO抑制激活素处理的动物帽的伸长和肌肉分化。第三，Chd-MO完全阻断了移植到宿主胚胎腹侧的组织者组织对中枢神经系统（CNS）、体节和脊索的诱导。出乎意料的是，移植到背侧揭示了神经板分化的脊索蛋白的细胞自主要求。
We analyzed the Chordin requirement in Xenopus development. Targeting of both chordin Xenopus laevis pseudoalleles with morpholino antisense oligomers (Chd-MO) markedly decreased Chordin production. Embryos developed with moderately reduced dorsoanterior structures and expanded ventroposterior tissues, phenocopying the zebrafish chordino mutant. A strong requirement for Chordin in dorsal development was revealed by experimental manipulations. First, dorsalization by lithium chloride treatment was completely blocked by Chd-MO. Second, Chd-MO inhibited elongation and muscle differentiation in Activin-treated animal caps. Third, Chd-MO completely blocked the induction of the central nervous system (CNS), somites, and notochord by organizer tissue transplanted to the ventral side of host embryos. Unexpectedly, transplantations into the dorsal side revealed a cell-autonomous requirement of Chordin for neural plate differentiation.