cDNA cloning and genomic organization of the mouse BMP type II receptor
cDNA cloning and genomic organization of the mouse BMP type II receptor
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DOI：
10.1006/bbrc.1997.6816
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发表时间：
1997-06-27
影响因子：
3.1
通讯作者：
Kawabata, M
中科院分区：
文献类型：
--
作者：
Beppu, H;Minowa, O;Kawabata, M
以人骨形态发生蛋白Ⅱ型受体（BMPR-II）为探针，分离出小鼠BMPR-II的cDNA，并测定了其基因组结构。该cDNA编码1，038个氨基酸，具有一个单一的跨膜结构域、一个丝氨酸/苏氨酸激酶结构域和一个长的羧基末端尾。小鼠和人BMPR-II之间的总体氨基酸序列同一性为96.6%。mRNA广泛分布于各种成体组织中。该基因由13个外显子编码，跨越80 kb。两个大的内含子（内含子1和3）贡献了大部分的基因大小，如在小鼠激活素II型受体基因。对内含子/外显子边界进行测序。结果表明，选择性剪接可以产生530个氨基酸的BMPR-II的较短形式，如先前所报道的。了解BMPR-II基因的结构对于了解骨形态发生蛋白在动物发育和生理过程中的作用至关重要。(C)北京：科学出版社.
The cDNA for the mouse bone morphogenetic protein type II receptor (BMPR-II) was isolated using the human counterpart as a probe and its genomic structure was determined, The cDNA encodes a protein of 1,038 amino acids with a single transmembrane domain, a serine/threonine kinase domain, and a long carboxy-terminal tail. The overall amino acid sequence identity between the mouse and the human BMPR-II is 96.6%. mRNA is widely distributed in various adult tissues. The gene is encoded by 13 exons spanning over 80 kb. Two large introns (intron 1 and 3) contribute to the majority of the gene size, as in the mouse activin type II receptor gene. The intron/exon boundaries were sequenced. The results suggest that alternative splicing can yield a shorter form of BMPR-II of 530 amino acids, as reported previously. Knowledge of the structure of the BMPR-II gene is essential for the understanding of the role of bone morphogenetic proteins in the developmental and physiological processes of animals. (C) 1997 Academic Press.