Characterization of EssB, a protein required for secretion of ESAT-6 like proteins in Staphylococcus aureus.
Characterization of EssB, a protein required for secretion of ESAT-6 like proteins in Staphylococcus aureus.
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DOI：
10.1186/1471-2180-12-219
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发表时间：
2012-09-25
期刊：
影响因子：
4.2
通讯作者：
Missiakas D
中科院分区：
文献类型：
--
作者：
Chen YH;Anderson M;Hendrickx AP;Missiakas D
金黄色葡萄球菌分泌EsxA和EsxB，这是WXG 100蛋白家族的两种小多肽。遗传分析表明，EsxA和EsxB的产生和分泌需要一个完整的ESAT-6分泌系统（ESS），这是一组在许多厚壁菌门中保守的基因，包括esxA和esxB。在这里，我们表征的ESTB，ESS集群编码的蛋白质之一。GTB在革兰氏阳性菌中高度保守，属于蛋白质COG 4499的邻位组簇，功能未知。通过在essB中产生一个内部缺失，我们证明了EsxA分泌所需的ESB。我们用多克隆抗体鉴定了SIBB，并表明该蛋白质与S的质膜分离。金黄色。然而，当在大肠杆菌中产生时，ESTB保持大部分可溶性，并且纯化的蛋白质组装成可以通过电子显微镜观察到的高度组织化的寡聚体。在野生型S.金黄色葡萄球菌赋予EsxA分泌显性阴性表型。这里提供的数据支持这样的观点，即在S中，WXG 100特异性的易位子可以寡聚化并与其他膜组分相互作用形成WXG 100。金黄色。
Staphylococcus aureus secretes EsxA and EsxB, two small polypeptides of the WXG100 family of proteins. Genetic analyses have shown that production and secretion of EsxA and EsxB require an intact ESAT-6 Secretion System (ESS), a cluster of genes that is conserved in many Firmicutes and encompasses esxA and esxB . Here, we characterize EssB, one of the proteins encoded by the ESS cluster. EssB is highly conserved in Gram-positive bacteria and belongs to the Cluster of Orthologous Groups of protein COG4499 with no known function. By generating an internal deletion in essB , we demonstrate that EssB is required for secretion of EsxA. We use a polyclonal antibody to identify EssB and show that the protein fractionates with the plasma membrane of S. aureus . Yet, when produced in Escherichia coli, EssB remains mostly soluble and the purified protein assembles into a highly organized oligomer that can be visualized by electron microscopy. Production of truncated EssB variants in wild-type S. aureus confers a dominant negative phenotype on EsxA secretion. The data presented here support the notion that EssB may oligomerize and interact with other membrane components to form the WXG100-specific translocon in S. aureus .
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影响因子：
3.6
作者：
Coros, Abbie;Callahan, Brian;Derbyshire, Keith M.
通讯作者：
Derbyshire, Keith M.
影响因子：
64.5
作者：
Economou, A;Pogliano, JA;Wickner, W
通讯作者：
Wickner, W
影响因子：
64.5
作者：
GORLICH, D;RAPOPORT, TA
通讯作者：
RAPOPORT, TA
DOI：
10.1073/pnas.0806338105
发表时间：
2008-10-07
影响因子：
11.1
作者：
Leake, Mark C.;Greene, Nicholas P.;Berks, Ben C.
通讯作者：
Berks, Ben C.
影响因子：
64.5
作者：
HARTL, FU;LECKER, S;WICKNER, W
通讯作者：
WICKNER, W