Potential epigenetic regulation of RNA 5′-terminal NAD decapping associated with cellular energy status of postharvest Fragaria x ananassa in response to Botrytis cinerea invasion
Potential epigenetic regulation of RNA 5′-terminal NAD decapping associated with cellular energy status of postharvest Fragaria x ananassa in response to Botrytis cinerea invasion
复制标题
RNA 5-末端 NAD 脱帽的潜在表观遗传调控与草莓采后响应灰霉病入侵的细胞能量状态相关
DOI：
10.1016/j.postharvbio.2022.111840
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发表时间：
2022-01-10
影响因子：
7
通讯作者：
Luo, Zisheng
中科院分区：
文献类型：
--
作者：
Ma, Quan;Xu, Yanqun;Luo, Zisheng
烟酰胺腺嘌呤二核苷酸(NAD)是一种重要的能量代谢产物，在植物免疫中起着信号物质的作用，现已成为RNA的5‘末端封顶。针对草莓对灰霉病的反应，研究了NAD去顶(DeNAD)与果实免疫力和能量状态之间的关系。随着病情的发展，草莓的能量状况逐渐下降，6d时持续下降至5.21+/-0.59mgkg(-)(1)，相应地，NAD含量达到158.25+/-22.5mmolkg(-1)，但在接下来的几天内下降。相反，NADPH和NADH的含量在接种后6d内下降。免疫基因对灰霉病和能量状态的改变有明显的反应。尤其是FaBIK1在6d的表达是1d的120倍，而FaCERK、FaPBL2和与NAD生物合成相关的基因可能受到病原菌的操纵和抑制。FaDXO1是已报道的唯一与deNAD相关的基因，在Fragaria物种中被鉴定，并且与拟南芥DXO1高度保守。作为对灰霉病的响应，FaDXO1在1d的表达比0d下调了3倍，但在接下来的接种阶段，能量状况的下降和灰霉病菌的感染都诱导了FaDXO1的表达。本研究首次报道了在草莓中发现的NAD解壳基因，并对其在灰霉病中的表达模式进行了研究。草莓采后脱NAD与病原菌侵染的关系与能量状态有关，揭示了RNA非规范NAD帽的生物学功能。
Nicotinamide adenine dinucleotide (NAD), a vital energy metabolite, functions as a signal substance in plant immunity, which has been emerged as a 5'-terminal capping of RNA. In response to gray mold disease, the association among NAD decapping (deNAD), fruit immunity and energy status was studied in strawberry. Obvious necrosis was observed at 3 d corresponded to the upregulation of BcActin A/FaActin at 2 d which was 18.9-fold than 1 d. With the development of disease, energy status of strawberry decreased with ATP content constantly decreased to 5.21 +/- 0.59 mg kg(-)(1) at 6 d. Accordingly, NAD content reached 158.25 +/- 22.5 mu mol kg(-1) but decreased at the following days of infection. Conversely, contents of NADPH and NADH were decreased during 6 days of inoculation. Immune genes expressed distinctly in response to gray mold and the change of energy status. Especially FaBIK1 expression in 6 d was 120-fold than in 1 d. However, FaCERK, FaPBL2 and genes related to NAD biosynthesis were presumably manipulated and suppressed by pathogen. FaDXO1, the only reported gene associated with deNAD was identified in Fragaria species and was highly conserved to Arabidopsis DXO1. In response to gray mold, expression of FaDXO1 in 1 d was downregulated 3-fold less than 0 d, but was induced by the decline of energy status and Botrytis cinerea infection at following stage of inoculation. This study first reported the identified NAD decapping genes in strawberry and evaluated its expression pattern in response to gray mold disease. The relationship between deNADing and pathogenic invasion of postharvest strawberry was linked by the energy status, which shed a light of the biological function of RNA noncanonical NAD cap.