Co-culture of osteoblasts and chondrocytes modulates cellular differentiation in vitro
Co-culture of osteoblasts and chondrocytes modulates cellular differentiation in vitro
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DOI：
10.1016/j.bbrc.2005.10.025
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发表时间：
2005-12-16
影响因子：
3.1
通讯作者：
Lu, HH
中科院分区：
文献类型：
--
作者：
Jiang, J;Nicoll, SB;Lu, HH
软骨移植与软骨下骨的生物整合仍然是一个重大的临床挑战。我们假设成骨细胞和软骨细胞之间的相互作用在组织工程化骨软骨移植物上骨软骨界面的再生中是重要的。我们在这里描述了一种序贯共培养模型，该模型允许三维培养中成骨细胞和软骨细胞之间的细胞-细胞接触和旁分泌相互作用。该模型被用来确定共培养对成骨细胞和软骨细胞表型维持的影响。研究发现，当软骨细胞合成II型胶原和糖胺多聚糖(GAG)基质时，共培养时GAG沉积明显减少。成骨细胞中碱性磷酸酶的活性保持不变，但共培养中细胞介导的矿化作用明显低于成骨细胞对照组。这些结果表明，成骨细胞和软骨细胞之间的相互作用调节细胞表型，这些相互作用对骨软骨界面再生的重要性将在未来的研究中得到探索。(C)2005 Elsevier Inc.保留所有权利。
Biological integration of cartilage grafts with subchondral bone remains a significant clinical challenge. We hypothesize that interaction between osteoblasts and chondrocytes is important in regenerating the osteochondral interface on tissue-engineered osteochondral grafts. We describe here a sequential co-culturing model which permits cell-cell contact and paracrine interaction between osteoblast and chondrocytes in 3-D culture. This model was used to determine the effects of co-culture on the phenotypic maintenance of osteoblasts and chondrocytes. It was found that while chondrocytes synthesized a type II collagen and glycosaminoglycan (GAG) matrix, GAG deposition was significantly lower in co-culture. Alkaline phosphatase activity was maintained in osteoblasts, but cell-mediated mineralization in co-culture was markedly lower compared to osteoblast controls. These results collectively suggest that interactions between osteoblasts and chondrocytes modulate cell phenotypes, and the importance of these interactions on osteochondral interface regeneration will be explored in future studies. (c) 2005 Elsevier Inc. All rights reserved.