URINARY METABOLITES OF L-THREONINE IN TYPE-1 DIABETES DETERMINED BY COMBINED GAS-CHROMATOGRAPHY CHEMICAL IONIZATION MASS-SPECTROMETRY
URINARY METABOLITES OF L-THREONINE IN TYPE-1 DIABETES DETERMINED BY COMBINED GAS-CHROMATOGRAPHY CHEMICAL IONIZATION MASS-SPECTROMETRY
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DOI：
10.1002/bms.1200131004
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发表时间：
1986-10-01
期刊：
影响因子：
--
通讯作者：
SWEELEY, CC
中科院分区：
文献类型：
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作者：
KASSEL, DB;MARTIN, M;SWEELEY, CC
青少年发病（1型）糖尿病患者的尿有机酸代谢谱显示2-羟基丁酸水平显著升高。为了检验这些代谢物以及4-脱氧阿糖胞苷磷酸来源于L-苏氨酸的假设，将稳定同位素标记的苏氨酸输注到胰岛素缺乏犬中，并通过气相色谱/质谱法监测13 C掺入这些代谢物中。电子电离是相对不敏感的，但正化学电离与氨作为反应气体得到质子化的分子和[M+ NH 4]+离子，这可以通过选择离子监测分析。观察到同位素标记的2-羟基丁酸、4-脱氧阿托膦酸和4-脱氧苏糖酸，但13 C未掺入其他有机酸中。因此，提出L-苏氨酸是这些代谢物的前体。
Metabolic profiling of urinary organic acids from patients with juvenile‐onset (Type 1) diabetes mellitus have revealed significantly elevated levels of 2‐hydroxybutyric acids. To test the hypothesis that these metabolites, as well as 4‐deoxyerythronic acid, are derived from L‐threonine, stable isotope‐labeled threonine was infused into an insulin‐deficient dog and the incorporation of13C into these metabolites was monitored by gas chromatography/mass spectrometry. Electron ionization was relatively insensitive, but positive chemical ionization with ammonia as the reactant gas gave both protonated molecules and [M+NH4]+ions, which could be analysed by selected ion monitoring. The isotope‐labeled species of 2‐hydroxybutyric, 4‐deoxyerythronic and 4‐deoxythreonic acids were observed, but13C was not incorporated into other organic acids. Thus, it is proposed that L‐threonine is a precursor of these metabolites.