Effect of cyanide on nitrovasodilator-induced relaxation, cyclic GMP accumulation and guanylate cyclase activation in rat aorta.
Effect of cyanide on nitrovasodilator-induced relaxation, cyclic GMP accumulation and guanylate cyclase activation in rat aorta.
复制标题
氰化物对硝基血管舒张剂诱导的大鼠主动脉舒张、环鸟苷酸积累和鸟苷酸环化酶激活的影响。
DOI：
10.1016/0014-2999(84)90369-8
复制
发表时间：
1984
影响因子：
5
通讯作者：
Murad,F
中科院分区：
文献类型：
--
作者：
Rapoport,RM;Murad,F
研究了氰化钠对硝普钠及其他硝基血管扩张剂所致大鼠胸主动脉舒张、环GMP积累和鸟苷酸环化酶激活的影响。氰化物消除硝普西引起的松弛和相关的环GMP水平的增加。环GMP和环AMP的基础水平也下降。硝普西诱导的弛豫逆转与加入氰化物前的组织环GMP水平无关。将硝普苷与氰化物孵育在主动脉条带上并没有改变硝普苷的松弛作用。叠氮化钠-、羟胺-、N-甲基-N '-硝基-N-亚硝基胍-、硝化甘油-和乙酰胆碱诱导的松弛和增加的环GMP水平也被氰化物抑制。由一氧化氮引起的松弛也被氰化物抑制，尽管低浓度一氧化氮引起的松弛不伴有可检测到的环GMP增加。氰化物对8-溴环GMP的弛豫基本没有影响；然而，异丙肾上腺素引起的松弛被抑制。在主动脉匀浆的可溶性和颗粒组分中，硝普苷激活了鸟苷酸环化酶，而氰化物则阻止了这种激活。目前的研究结果表明，氰化物通过抑制鸟苷酸环化酶的激活来抑制硝基血管扩张剂诱导的松弛；然而，氰化物也可能具有抑制松弛的非特异性作用。
The effects of sodium cyanide on relaxation, increases in cyclic GMP accumulation and guanylate cyclase activation induced by sodium nitroprusside and other nitrovasodilators were examined in rat thoracic aorta. Cyanide abolished nitroprusside-induced relaxation and the associated increase in cyclic GMP levels. Basal levels of cyclic GMP and cyclic AMP were also depressed. Reversal of nitroprusside-induced relaxation by cyanide was independent of the tissue level of cyclic GMP prior to addition of cyanide. Incubation of nitroprusside with cyanide prior to addition to aortic strips did not alter the relaxant effect of nitroprusside. Sodium azide-, hydroxylamine-, N-methyl- N'-nitro-N-nitrosoguanide-, nitroglycerin- and acetylcholine-induced relaxations and increased levels of cyclic GMP were also inhibited by cyanide. Relaxations induced by nitric oxide were also inhibited by cyanide, although the relaxation with the low concentration of nitric oxide employed was not accompanied by detectable increases in cyclic GMP. Relaxation to 8-bromo-cyclic GMP was essentially unaltered by cyanide; however, isoproterenol-induced relaxation was inhibited. Guanylate cyclase in soluble and particulate fractions of aorta homogenates was activated by nitroprusside and the activation was prevented by cyanide. The present results suggest that cyanide inhibits nitrovasodilator-induced relaxation through inhibition of guanylate cyclase activation; however, cyanide may also have nonspecific effects which inhibit relaxation.