[Multicolor fluorescence in situ hybridization (mFISH].
[Multicolor fluorescence in situ hybridization (mFISH].
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DOI：
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发表时间：
2001-03
影响因子：
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通讯作者：
K. Michalová;Z. Zemanová;J. Březinová
中科院分区：
文献类型：
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作者：
K. Michalová;Z. Zemanová;J. Březinová
现在有各种技术可用于基因和DNA序列的拷贝数、结构和表达的改变的广泛基因组筛选。分子细胞遗传学有比较基因组杂交（CGH）、光谱核型分析（SKY）和荧光原位杂交（mFISH）等技术。我们目前的原则，这些方法，其使用的分子细胞遗传学检查的病人。我们引用我们的经验与mFISH分析复杂的染色体重排在肿瘤细胞和鉴定先天性额外标记染色体。此外，我们还回顾了染色体高分辨显带（mBAND）的首次经验。应用该方法对骨髓增生异常综合征伴5号染色体缺失患者的骨髓细胞进行了分析。用mBAND定位精确的断点。多色荧光方法mFISH和mBAND成为更精确地分析先天性和获得性染色体数目和结构重排的新工具。
Various techniques are now available for wide genome screening of alterations in copy number, structure and expression of genes and DNA sequences. Molecular cytogenetics has special techniques of comparative genomic hybridization (CGH), spectral karyotyping (SKY) and multicolor FISH (mFISH). We present principles of these methods, their use in molecular cytogenetic examinations of patients. We quote our experience with mFISH for analyses of complex chromosomal rearrangements in neoplastic cells and identification of inborn supernumerary marker chromosome. Further, we also review the first experiences with multicolor high resolution banding of chromosome (mBAND). This method was used for analysis of bone marrow cells of patient with myelodysplastic syndrome and deletion of chromosome No. 5. With mBAND exact breakpoints were localized. Multicolor fluorescence methods mFISH and mBAND becones the new tools for more precise analyses of inborn and acquired numerical and structural chromosomal rearrangements.