Protective Effects of Ascorbate and Catalase on Human Spermatozoa During Cryopreservation
Protective Effects of Ascorbate and Catalase on Human Spermatozoa During Cryopreservation
复制标题
抗坏血酸和过氧化氢酶对人精子冷冻保存的保护作用
DOI：
10.2164/jandrol.109.007849
复制
发表时间：
2010-09-01
影响因子：
--
通讯作者：
Liu, Wanmin
中科院分区：
文献类型：
--
作者：
Li, Zhiling;Lin, Qionglin;Liu, Wanmin
人类精子冷冻保存在男性不育的临床治疗中存在精子冷冻损伤的问题。我们旨在阐明冷冻损伤的完整模式，并评估抗坏血酸和过氧化氢酶对冷冻保存精子的保护作用。采集了30名育龄男性的精液样本。每个样本分为6组：新鲜精液，未经处理的冷冻保存精液，抗坏血酸（300或600 μ M）或过氧化氢酶（200或400 IU/mL）冷冻保存的样本。在低温保存期间，检测精子在抗坏血酸或过氧化氢酶存在或不存在的情况下的活力、运动性、活性氧（ROS）生成、线粒体膜电位（MMP）、凋亡（膜联蛋白V阳性、碘化丙啶阴性[即Ann(+)/ PI-]）和DNA损伤（橄榄尾矩[OTM]）。与新鲜精子相比，冷冻保存精子的活力、活力和MMP显著降低，而Ann(+)/PI-和OTM显著升高（P < 0.01和P < 0.01）
Human semen cryopreservation in the clinical management of male infertility is complicated by cryodamage to spermatozoa. We aimed to clarify the full pattern of cryodamage and evaluate the protective effects of ascorbate and catalase on cryopreserved spermatozoa. Semen samples were collected from 30 fertile males. Each sample was divided into 6 groups: fresh semen, cryopreserved semen without treatment, and samples cryopreserved with ascorbate (300 or 600 mu M) or catalase (200 or 400 IU/mL). Spermatozoa were examined for their viability, motility, reactive oxygen species (ROS) generation, mitochondrial membrane potential (MMP), apoptosis (positive for annexin V and negative for propidium iodide [ie, Ann(+)/ PI-]), and DNA damage (Olive tail moment [OTM]) in the presence or absence of ascorbate or catalase during cryopreservation. In comparison with the fresh spermatozoa, there was a significant decrease in the viability, motility, and MMP but increase in Ann(+)/PI- and OTM in the cryopreserved spermatozoa (P < .01 and P