Calbindin D28k expression in immunohistochemically identified Renshaw cells
Calbindin D28k expression in immunohistochemically identified Renshaw cells
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DOI：
10.1097/00001756-199808030-00043
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发表时间：
1998-08-03
期刊：
影响因子：
1.7
通讯作者：
Fyffe, REW
中科院分区：
文献类型：
--
作者：
Carr, PA;Alvarez, FJ;Fyffe, REW
双免疫荧光法检测Renshaw细胞是否具有calbindin D28k免疫反应性。利用大鼠和猫腰椎脊髓切片中gephyrin免疫反应性的特征性表达模式对Renshaw细胞进行鉴定。在大鼠中，所有归为Renshaw细胞的神经元（n = 487）均含有calbindin d28k免疫反应性，且位于第七层最腹侧区域的calbindin d28k免疫反应性细胞均表达Renshaw细胞特有的gephyrin标记和形态。在猫中，只有不到一半的Renshaw细胞（47%;n = 128）被双重标记。在这两个物种中，偶有calbindin d28k免疫反应的Renshaw细胞在IX层的运动核中被发现。Renshaw细胞独特的免疫标记使我们估计在大鼠第四腰椎节段的每个前角约有250个Renshaw细胞，在猫的L6节段的每个前角约有750个Renshaw细胞。我们得出结论，Renshaw细胞的功能特性，包括其高速率激发动作电位的能力，可能需要特定的稳态机制，包括强细胞内钙缓冲，其精确机制可能因物种而异。(C) 1998快速科学有限公司
DOUBLE immunofluorescence was utilized to determine whether Renshaw cells contain calbindin D28k immunoreactivity. Renshaw cells were identified by their characteristic expression patterns of gephyrin immunoreactivity in sections of rat and cat lumbar spinal cord. In the rat, all neurons classified as Renshaw cells (n = 487) also contained calbindin D28k-immunoreactivity, and all calbindin D28k-immunoreactive cells located in the ventral-most region of lamina VII expressed the characteristic gephyrin labeling and morphology of Renshaw cells. In the cat, fewer than half of the Renshaw cells (47%; n = 128) were double-labeled. In both species, occasional calbindin D28k-immunoreactive Renshaw cells were identified within motor nuclei in lamina IX. The distinctive immunolabeling of Renshaw cells allowed us to estimate that there are about 250 Renshaw cells in each ventral horn of the fourth lumbar segment of rat spinal cord, and about 750 cells per ventral horn in the L6 segment of the cat. We conclude that the functional properties of Renshaw cells, including their ability to fire action potentials at high rates, likely require specific homeostatic mechanisms including strong intracellular calcium buffering, the precise mechanisms of which may vary between species. (C) 1998 Rapid Science Ltd.