Fibrillin-1 (FBN-1) a new marker of germ cell neoplasia in situ.
Fibrillin-1 (FBN-1) a new marker of germ cell neoplasia in situ.
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DOI：
10.1186/s12885-016-2644-z
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发表时间：
2016-08-04
期刊：
影响因子：
3.8
通讯作者：
Babal P
中科院分区：
文献类型：
--
作者：
Cierna Z;Mego M;Jurisica I;Machalekova K;Chovanec M;Miskovska V;Svetlovska D;Kalavska K;Rejlekova K;Kajo K;Mardiak J;Babal P
原位生殖细胞瘤（GCNIS）是睾丸生殖细胞肿瘤（TGCTs）的侵袭前阶段。纤维蛋白是微原纤维的组成部分，被认为参与了癌症的发病和胚胎干细胞多能性的维持。本研究的目的是检测tgct患者中纤维蛋白-1 （FBN-1）的表达。203例tgct患者的手术标本被纳入转化研究。FBN-1在所有可用病例的肿瘤组织、GCNIS和邻近非肿瘤性睾丸组织中表达。组织样品采用组织微阵列法处理。采用山羊多克隆抗体免疫组化检测FBN-1，并采用乘法快速评分法（QS）评价其表达。FBN-1阳性在GCNIS中最高（平均QS = 11.30），大多数病例（77.1%）FBN-1过表达（QS bbb9）。FBN-1在所有tgct亚型中的表达均显著低于GCNIS中的表达（均p <0.001）。精原细胞瘤的表达与EC、ChC和TER比较，差异均有统计学意义（p <0.05），与YST比较，差异无统计学意义（p = 0.84）。非肿瘤性睾丸组织FBN-1阳性极低（平均QS = 0.02）。FBN-1表达对GCNIS诊断的敏感性、特异性、阳性预测值和阴性预测值分别为97.1、98.8%、98.6%和97.7%。与生殖细胞肿瘤患者的非肿瘤性睾丸组织相比，FBN-1在tgct中过度表达，特别是在GCNIS中，可能参与生殖细胞原位肿瘤的发展。
Germ cell neoplasia in situ (GCNIS), is preinvasive stage of testicular germ cell tumours (TGCTs). Fibrillins, which are integral components of microfibrils are suggested to be involved in cancer pathogenesis and maintenance of embryonic stem cells pluripotency. The aim of this study was to examine fibrillin-1 (FBN-1) expression in TGCTs patients. Surgical specimens from 203 patients with TGCTs were included into the translational study. FBN-1 expression was evaluated in the tumour tissue, in GCNIS and in adjacent non-neoplastic testicular tissue in all available cases. Tissue samples were processed by the tissue microarray method. FBN-1 was detected by immunohistochemistry using goat polyclonal antibody and the expression was evaluated by the multiplicative quickscore (QS). The highest FBN-1 positivity was detected in GCNIS (mean QS = 11.30), with overexpression of FBN-1 (QS >9) in the majority (77.1 %) of cases. Expression of FBN-1 in all subtypes of TGCTs was significantly lower in comparison to expression in GCNIS (all p <0.001). Seminoma had significantly higher expression compared to EC, ChC and TER (all p <0.05), but not to YST (p = 0.84). In non-neoplastic testicular tissue the FBN-1 positivity was very low (mean QS = 0.02). Sensitivity, specificity, positive and negative predictive value of FBN-1 expression for diagnosis of GCNIS were 97.1, 98.8, 98.6 and 97.7 %. FBN-1 is overexpressed in TGCTs and especially in GCNIS when compared to non-neoplastic testicular tissue in patients with germ cell tumors and could be involved in germ cell neoplasia in situ development.