A SOIL TOXICITY TEST USING THE NEMATODE CAENORHABDITIS-ELEGANS AND AN EFFECTIVE METHOD OF RECOVERY
A SOIL TOXICITY TEST USING THE NEMATODE CAENORHABDITIS-ELEGANS AND AN EFFECTIVE METHOD OF RECOVERY
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DOI：
10.1007/bf00212125
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发表时间：
1993-08-01
影响因子：
4
通讯作者：
DUSENBERY, DB
中科院分区：
文献类型：
--
作者：
DONKIN, SG;DUSENBERY, DB
一种新的方法回收线虫从土壤中的一个有效的，可重复的，和非破坏性的方式已经开发出来。它被用来进行短期的土壤毒性测试，使用土居线虫秀丽隐杆线虫和几种不同的土壤类型与氯化铜加标。回收方法涉及通过胶体二氧化硅悬浮液离心，允许从土壤基质中提取线虫，以便评估致死率。线虫是无害的恢复程序，活的和死的个人都恢复了高效率（远超过80%），允许重现的浓度-响应曲线后，24小时的曝光。土壤的存在使铜的LC 50增加了约10倍，不同土壤对毒性的影响差异显著。铜离子的毒性还受测试溶液中氯化钠和氯化钾浓度的影响，细菌的存在增加了某些土壤中铜离子的毒性。土壤中的LC 50接近2周土壤毒性试验的LC 50，表明24小时线虫毒性试验在敏感性方面可能与2周土壤毒性试验相当。
A new method for recovering nematodes from soils in an efficient, reproducible, and non-destructive manner has been developed. It was used to conduct short-term soil toxicity tests using the soil-dwelling nematode Caenorhabditis elegans and several different soil types spiked with copper chloride. The recovery method, which involves centrifugation through a colloidal silica suspension, allows the nematodes to be extracted from the soil matrix so that lethality can be assessed. The nematodes are unharmed by the recovery procedure, and both live and dead individuals are recovered with high efficiency (well over 80%), allowing reproducible concentration-response curves to be made after a 24-h exposure. The LC50s for copper were increased about tenfold by the presence of soil, and different soils had significantly different effects on toxicity. Toxicity of copper ion was also influenced by the concentration of sodium chloride and potassium chloride in the test solution, and the presence of bacteria increased the toxicity of copper ion in some soils. The LC50s in soil were close to the LC50 for the 2-week earthworm soil toxicity test, suggesting that a 24-h nematode toxicity test may be comparable to the 2-week earthworm test in terms of sensitivity.