Protein interaction of retinoblastoma gene product pRb110 with M(r) 73,000 heat shock cognate protein.
Protein interaction of retinoblastoma gene product pRb110 with M(r) 73,000 heat shock cognate protein.
复制标题
视网膜母细胞瘤基因产物 pRb110 与 M(r) 73,000 热休克同源蛋白的蛋白质相互作用。
作者：
T. Nihei;S. Takahashi;S. Sagae;N. Sato;K. Kikuchi
两种肿瘤抑制基因产物，视网膜母细胞瘤敏感性基因产物pRb 110和p53，都与DNA肿瘤病毒的癌基因产物形成寡聚体复合物。已经证明p53与M（r）70，000热休克蛋白家族结合。然而，pRb 110与M（r）70，000热休克蛋白家族的蛋白关联尚不清楚。我们分析了TYK-nu人卵巢癌细胞裂解物与抗pRb 110或抗热休克蛋白单克隆抗体的免疫沉淀。在本文中，我们证明了pRb 110与M（r）73，000热休克同源蛋白相关，但与M（r）72，000热休克蛋白无关。这种选择性的蛋白质结合在HeLa宫颈癌细胞中也被检测到。此外，M（r）73，000热休克同源蛋白和pRb 110的蛋白质复合物在ATP的存在下解离，但在ADP和不可水解的ATP类似物ATP gamma S的存在下不解离。这表明解离依赖于ATP水解。这些数据可能表明M（r）73，000热休克同源蛋白与pRb 110协同作用在细胞生长控制中的重要作用尚未确定。
Both the tumor suppressor gene products, the retinoblastoma sensitivity gene product pRb110 and p53, are found in oligomer complexes with the oncogene products of the DNA tumor viruses. It has been demonstrated that p53 binds to the M(r) 70,000 heat shock protein family. However, the protein association of pRb110 with the M(r) 70,000 heat shock protein family is not yet known. We analyzed the immunoprecipitates made with TYK-nu human ovarial carcinoma cell lysate and anti-pRb110 or anti-heat shock protein monoclonal antibodies. In this paper, we demonstrate that pRb110 is associated with the M(r) 73,000 heat shock cognate protein, but not with the M(r) 72,000 heat shock protein. This selective protein association was also detected in HeLa cervical carcinoma cells. Furthermore, the protein complexes of the M(r) 73,000 heat shock cognate protein and pRb110 were dissociated with the presence of ATP, but not with ADP and the nonhydrolyzable ATP analogue, ATP gamma S. This indicates that the dissociation is dependent on the ATP hydrolysis. These data may suggest an as yet undefined important role of M(r) 73,000 heat shock cognate protein in the cell growth control in collaboration with pRb110.
影响因子：
56.9
作者：
FLYNN, GC;CHAPPELL, TG;ROTHMAN, JE
通讯作者：
ROTHMAN, JE
DOI：
10.1073/pnas.88.13.5719
发表时间：
1991-07-01
影响因子：
11.1
作者：
PALLEROS, DR;WELCH, WJ;FINK, AL
通讯作者：
FINK, AL
视网膜母细胞瘤基因表达的稳定性决定了重建的视网膜母细胞瘤细胞的致瘤性。
DOI：
--
发表时间：
1992
期刊：
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research
影响因子：
--
作者：
Chen,PL;Chen,Y;Shan,B;Bookstein,R;Lee,WH
通讯作者：
Lee,WH