OMIP-084: 28-color full spectrum flow cytometry panel for the comprehensive analysis of human γδ T cells
OMIP-084: 28-color full spectrum flow cytometry panel for the comprehensive analysis of human γδ T cells
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DOI：
10.1002/cyto.a.24564
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发表时间：
2022-05-06
期刊：
影响因子：
3.7
通讯作者：
Prinz, Immo
中科院分区：
文献类型：
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作者：
Barros-Martins, Joana;Bruni, Elena;Prinz, Immo
使用全光谱流式细胞术，我们设计了一个28色面板，用于分析已知与γ δ T细胞免疫反应相关的标记物。该面板允许通过其T细胞受体（TCR）的特定V基因使用（V γ 9, V δ 1， V δ 2和V δ 3）并根据其功能分化对γ δ T细胞亚群进行分类。区分细胞成熟不同阶段的表型表面受体包括CD45RA、CD27、CD28、CD127、CD57和CD16；趋化因子受体CXCR6、CCR5、CCR6和CX3CR1；nk相关标记物NKG2A， NKG2D， CD56和CD161，检查点抑制剂PD-1，以及激活受体CD38和CD25。还包括用于分析α - β T细胞（CD4和CD8）和MATT细胞（V α 7.2）的T细胞谱系标记。这种优化的多色面板允许对所有主要的人类γ δ T细胞亚群进行全面的免疫分析，适用于临床队列中γ δ T细胞发育和γ δ T细胞动力学的纵向或探索性分析。
Using full spectrum flow cytometry, we designed a 28-color panel for the analysis of markers known to be associated with the gamma delta T cell immune response. This panel allows the classification of gamma delta T cell subsets via specific V gene usage (V gamma 9, V delta 1, V delta 2, and V delta 3) of their T cell receptor (TCR) and according to their functional differentiation. Phenotypical surface receptors to distinguish different stages of cell maturation included CD45RA, CD27, CD28, CD127, CD57, and CD16; chemokine receptors CXCR6, CCR5, CCR6, and CX3CR1; NK-associated markers NKG2A, NKG2D, CD56, and CD161, checkpoint-inhibitor PD-1, and activating receptors CD38 and CD25. T cell lineage markers for the analysis of alpha beta T cells (CD4 and CD8) and MATT cells (V alpha 7.2) were also included. This optimized multicolor panel allows a comprehensive immune-profiling of all main human gamma delta T cell subsets and is suitable for longitudinal or exploratory analysis of gamma delta T cell development and gamma delta T cell dynamics in clinical cohorts.