Facile preparation of complex protein architectures with sub-100-nm resolution on surfaces
Facile preparation of complex protein architectures with sub-100-nm resolution on surfaces
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DOI：
10.1002/anie.200700989
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发表时间：
2007-01-01
影响因子：
16.6
通讯作者：
Delamarche, Emmanuel
中科院分区：
文献类型：
--
作者：
Coyer, Sean R.;Garcia, Andres J.;Delamarche, Emmanuel
表面上的蛋白质在宿主对植入生物医学器械的反应和生物技术应用中发挥着普遍和核心的作用，[1]包括体外基于表面的诊断测定[2]和细胞培养支持。[3]在许多情况下，复杂的生物功能是由多种类型蛋白质的相互作用引起的：例如，在涉及抗原呈递细胞的免疫反应中，[4]在骨再生中，[5]和在细胞粘附中。[6]这些系统的活动特别依赖于主要发生在纳米尺度上的空间组织。这刺激了新型生物启发材料和纳米纤维路线的发展。[7-9]因此，控制蛋白质图案化的能力不仅对深入了解生物现象很重要，[10]而且也是高性能生物传感器[11，12]和新型制造模式的先决条件。[13]许多方法已经被用于以高分辨率在表面上图案化蛋白质，包括蘸笔光刻，[14]微接触印刷，[15-19]自组装，[20]使用各种技术将图案烧蚀成蛋白质或有机分子的单层，[21]和基于扫描探针方法的纳米接枝。[22]尽管有这些努力，由于实际的限制，没有一种技术被广泛应用于研究生物现象中表面蛋白质的作用。这些限制包括在大面积上高通量生产具有纳米级特征的样品所需的时间，需要特定的表面化学将蛋白质从溶液吸附到表面上，以及需要在表面上形成纳米颗粒。
Proteins on surfaces play a ubiquitous and central role in host responses to implanted biomedical devices and in biotechnological applications,[1] including in vitro surface-based diagnostic assays [2] and cell-culture supports.[3] In many cases, complex biological functionality results from the interplay of multiple types of proteins: for example, in immune responses involving antigen-presenting cells,[4] in bone regeneration,[5] and in cell adhesion.[6] The activity of these systems is particularly dependent on a spatial organization that occurs primarily on the nanoscale. This has spurred the development of novel bioinspired materials and of nanofabrication routes.[7–9] The ability to control the patterning of proteins is, therefore, not only important for gaining insight into biological phenomena,[10] but is also a prerequisite for highperformance biosensors [11, 12] and novel fabrication paradigms.[13]Many approaches have been pursued for patterning proteins on surfaces with high resolution, including dip–pen lithography,[14] microcontact printing,[15–19] self-assembly,[20] ablation of patterns into monolayers of proteins or organic molecules using various techniques,[21] and nanografting based on scanning-probe methods.[22] Despite these efforts, no single technique has been widely applied to investigate the role of proteins on surfaces in biological phenomena because of practical limitations. These limitations include the time required for the high-throughput production of samples with nanoscale features over large areas, the need for specific surface chemistry to adsorb proteins from solution onto