Immunocytochemical localization of prostaglandin EP3 receptor in the rat hypothalamus
Immunocytochemical localization of prostaglandin EP3 receptor in the rat hypothalamus
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DOI：
10.1016/s0304-3940(98)00962-8
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发表时间：
1999-01-29
影响因子：
2.5
通讯作者：
Negishi, M
中科院分区：
文献类型：
--
作者：
Nakamura, K;Kaneko, T;Negishi, M
产生针对大鼠前列腺素 EP3 受体 (EP3R) N 末端部分的兔抗体，以检查 EP3R 在大鼠下丘脑中的分布。该抗体特异性识别大鼠脑提取物、大鼠肾膜组分以及表达 EP3R 的培养细胞膜组分中的 EP3R 蛋白。在正中视前核、内侧视前区、旁房核、下丘脑背内侧核的致密部分和前乳头核的背侧部分观察到强烈的EP3R样免疫反应性。这些结果表明前列腺素 Ep 介导下丘脑的各种作用，例如通过 EP3R 在视前区诱导发热。 (C) 1999 Elsevier Science ireland Ltd. 保留所有权利。
A rabbit antibody against an N-terminal portion of rat prostaglandin EP3 receptor (EP3R) was produced to examine the distribution of EP3R in the rat hypothalamus. The antibody specifically recognized EP3R proteins in rat brain extract, in membrane fractions of rat kidney, and in membrane fractions of EP3R-expressing culture cells. Intense EP3R-like immunoreactivity was observed in the median preoptic nucleus, medial preoptic area, parastrial nucleus, compact part of the dorsomedial hypothalamic nucleus, and dorsal part of the premammillary nucleus. These results suggest that prostaglandin Ep mediates various actions in the hypothalamus, such as fever induction in the preoptic area, through EP3R. (C) 1999 Elsevier Science ireland Ltd. All rights reserved.