Influence of plant ontogeny and abiotic factors on resistance of glandular-haired alfalfa to potato leafhopper (Hemiptera: Cicadellidae)
Influence of plant ontogeny and abiotic factors on resistance of glandular-haired alfalfa to potato leafhopper (Hemiptera: Cicadellidae)
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DOI：
10.1603/0022-0493-99.2.537
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发表时间：
2006-04-01
影响因子：
2.2
通讯作者：
Johnson, DW
中科院分区：
文献类型：
--
作者：
Casteel, CL;Ranger, CM;Johnson, DW
通过室内试验研究了腺毛苜蓿（Medicago sativa L.）对抗马铃薯叶蝉Empoasca fabae（Harris）。在叶蝉抗性植株内的变异性进行了检查，无论是对叶蝉抗性，腺毛M的基部或顶端茎节间的叶蝉成虫笼。sativa基因型G98 A或感病的非腺毛M. "流浪者“年轻的，积极分泌腺毛位于顶端节间的G98 A，而衰老的腺头被发现在老年人，基部节间的G98 A。96小时后，最高的累积叶蝉死亡率和最低数量的排泄液滴与G98 A的顶端节间。在G98 A的基部节间和Ranger的基部和顶部节间上，昆虫的死亡率和取食水平没有差异。通过在4种不同的低、强光（250和1,000 μ mol·s ~（-1）m ~（-2））和温度（17和30 ℃）条件下，用G98 A或Ranger对叶蝉成虫进行笼养，研究了非生物因子对叶蝉抗性的影响。96小时后，最高的累积死亡率与叶蝉局限于G98 A在强光和高温条件下。温度水平和植物类型也有影响的排泄液滴的生产，导致在最高数量的排泄液滴与游侠在高温制度。这些结果表明，M. Sativa G98 A对马铃薯叶蝉的保护作用优于其它品种，温度影响腺毛苜蓿的抗性水平。
Laboratory experiments were conducted to characterize the trichome-based defense of glandular-haired alfalfa, Medicago sativa L., against the potato leafhopper, Empoasca fabae (Harris). Within-plant variability in leafhopper resistance was examined by caging adult leafhoppers to either basal or apical stem internodes of the leafhopper-resistant, glandular-haired M. sativa genotype G98A or the susceptible, nonglandular-haired M. sativa 'Ranger'. Young, actively secreting glandular trichomes are located on apical internodes of G98A, whereas senesced gland heads are found on older, basal internodes of G98A. After 96 h, the highest cumulative leafhopper mortality and lowest number of excretory droplets were associated with apical internodes of G98A. No difference was detected in mortality and feeding levels among insects caged to basal internodes of G98A and basal and apical internodes of Ranger. The influence of abiotic factors on leafhopper resistance was evaluated by caging adult leafboppers to either G98A or Ranger under four combinations of low and high light (250 and 1,000 mu mol s(-1) m(-2)) and temperature regimes (17 and 30 degrees C). After 96 h, the highest cumulative mortality was associated with leafhoppers confined to G98A under high light and high temperature conditions. Temperature level and plant type also had an effect on the production of excretory droplets, resulting in the highest number of excretory droplets being associated with Ranger under the high temperature regime. These results indicate that certain regions of M. sativa G98A are better protected against the potato leafhopper than others and that temperature influences resistance levels of glandular-haired alfalfa.