Glucose oxidase anode for biofuel cell based on direct electron transfer
Glucose oxidase anode for biofuel cell based on direct electron transfer
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DOI：
10.1016/j.elecom.2006.05.024
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发表时间：
2006-08-01
影响因子：
5.4
通讯作者：
Apblett, Christopher
中科院分区：
文献类型：
--
作者：
Ivnitski, Dmitri;Branch, Brittany;Apblett, Christopher
本文提出了一种基于酶活性位点与多壁碳纳米管修饰电极表面之间的直接电子转移（DET）的葡萄糖氧化酶（GO(x)）电极作为生物燃料电池阳极的新设计概念。以多孔三维网络（78%孔隙率）的东丽(R)碳纸（TP）为基质，选择性生长多壁碳纳米管。通过化学气相沉积技术，在钴金属种子的电化学转变后，将MWCNTs并入TP中。这种方法能够有效地促进DET反应。采用扫描电镜、循环伏安法和电位法研究了氧化石墨烯修饰电极的形貌和电化学特性。聚阳离子聚乙烯亚胺（PEI）与带负电荷的葡萄糖氧化酶的结合在TP/MWCNT表面形成了类似于100 nm厚的薄膜。采用四丁基溴化铵盐处理过的Nafion (R)作为氧化石墨烯(x)的粘结剂和质子传导介质。TP/MWCNT/PEI/GO(x)/Nafion (R)修饰电极在含有0.1 M KCl的0.02 M磷酸盐缓冲溶液（pH 6.9）中在20 mM葡萄糖存在下在25℃下工作。GO(x)阳极的开路电位相对于Ag/AgCl在-0.38 ~ -0.4 V之间，更接近于酶本身的FAD/FADH(2)辅因子的氧化还原电位。氧化石墨烯(x)电极具有利用内源性物质（如葡萄糖和氧气）在体内工作的潜力。这种葡萄糖阳极允许开发新一代小型化无膜生物燃料电池。(c) 2006 Elsevier B.V.版权所有
This paper presents a new design concept of a glucose oxidase (GO(x)) electrode as an anode for the biofuel cell based on direct electron transfer (DET) between the active site of an enzyme and the multi-walled carbon nanotube (MWNT)-modified electrode surface. Toray (R) carbon paper (TP) with a porous three-dimensional network (78% porosity) was used as a matrix for selectively growing multi-walled carbon nanotubes. The incorporation of MWCNTs into TP was provided by the chemical vapor deposition technique after an electrochemical transition of cobalt metal seeds. This approach has the ability to efficiently promote DET reactions. The morphologies and electrochemical characteristics of the GO(x) modified electrodes were investigated by scanning electron microscopy, cyclic voltammetry, and potentiometric methods. The combination of poly-cation polyethylenimine (PEI) with negatively charged glucose oxidase provides formation of similar to 100 nm thick films on the TP/MWCNT surface. The tetrabutylammonium bromide salt-treated Nafion (R) was used as GO(x) binder and proton-conducting medium. The TP/MWCNT/PEI/GO(x)/Nafion (R) modified electrode operates at 25 degrees C in 0.02 M phosphate buffer solution (pH 6.9) containing 0.1 M KCl in the presence of 20 mM glucose. The open circuit potential of GO(x) anode was between -0.38 V and -0.4 V vs. Ag/AgCl, which is closer to the redox potential of the FAD/FADH(2) cofactor in the enzyme itself. The GO(x) electrode has a potential to work in vivo by using endogenous substances, such as glucose and oxygen. Such a glucose anode allows for the development of a new generation of miniaturized membrane-less biofuel cells. (c) 2006 Elsevier B.V. All rights reserved.