Isolation and characterization of Vsx1, a novel mouse CVC paired-like homeobox gene expressed during embryogenesis and in the retina.
Isolation and characterization of Vsx1, a novel mouse CVC paired-like homeobox gene expressed during embryogenesis and in the retina.
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DOI：
10.1006/bbrc.2001.5372
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发表时间：
2001-08
影响因子：
3.1
通讯作者：
A. Ohtoshi;M. Justice;R. Behringer
中科院分区：
文献类型：
--
作者：
A. Ohtoshi;M. Justice;R. Behringer
利用简并RT-PCR技术从小鼠原肠胚期胚胎RNA中筛选到一个新的类配对同源盒基因。该cDNA编码的开放阅读框与人VSX 1最相似。小鼠Vsx 1编码363个氨基酸残基的蛋白质，其含有CVC结构域，该结构域最初被鉴定为小鼠CHX 10、金鱼VSX-1和C. elegans CEH-10.连锁分析表明，小鼠Vsx 1定位于2号染色体的远端区域。RT-PCR分析检测到小鼠Vsx 1的成绩单从原肠胚和原肠胚后阶段的小鼠胚胎，表明Vsx 1在小鼠胚胎发生过程中的作用。对用胚胎干细胞产生的小鼠嵌合体的眼睛进行分析，其中lacZ报告基因靶向Vsx 1位点，表明Vsx 1在视网膜的内核层中表达。
Gastrula stage mouse embryo RNA was screened by degenerate RT-PCR to yield a novel paired-like homeobox gene. The open reading frame encoded by the cDNA was most similar to human VSX1. Mouse Vsx1 encodes a protein of 363 amino acid residues that contains a CVC domain that was originally identified as a conserved motif among mouse CHX10, goldfish VSX-1 and C. elegans CEH-10. Linkage analysis showed that mouse Vsx1 mapped to the distal region of chromosome 2. RT-PCR analysis detected mouse Vsx1 transcripts from gastrulation and post-gastrulation stage mouse embryos, suggesting a role for Vsx1 during mouse embryogenesis. Analysis of the eyes of mouse chimeras generated with embryonic stem cells in which a lacZ reporter was targeted to the Vsx1 locus suggested that Vsx1 is expressed in the inner nuclear layer of the retina.