Changes in biomechanical properties, composition of collagen and elastin, and advanced glycation endproducts of the rat aorta in relation to age
Changes in biomechanical properties, composition of collagen and elastin, and advanced glycation endproducts of the rat aorta in relation to age
复制标题
DOI：
10.1016/s0021-9150(96)05947-3
复制
发表时间：
1996-12-20
期刊：
影响因子：
5.3
通讯作者：
Oxlund, H
中科院分区：
文献类型：
--
作者：
Bruel, A;Oxlund, H
在老化和衰老过程中，主动脉变得更硬，其弹性降低。使用大鼠模型研究了导致主动脉壁刚度增加的机制。从4.5、14和27月龄的三组大鼠（代表年轻、成年和老年动物）的胸主动脉制备环形样品。静态生物力学特性分析显示，与成年大鼠相比（分别为1.82 +/-0.02 mm和3.0 +/- 0.1 mN），老年大鼠主动脉样本的直径（2.20 +/-0.03 mm）和刚度（4.0 +/-0.2 mN）增加。每个样品的总羟脯氨酸和弹性蛋白含量没有变化。但与成年大鼠相比，老年大鼠主动脉壁羟脯氨酸含量/mm（2）减少了20%，弹性蛋白含量/mm（2）减少了19%。未发现老年和成年大鼠之间的吡啶啉浓度的差异。胶原蛋白和弹性蛋白相关的荧光被确定为晚期糖基化终产物（AGE）的标志物。与成年大鼠相比，老年大鼠的这两个参数分别增加了42%和17%，并且与生理负荷下的刚度正相关。胶原相关荧光和最大硬度之间也呈正相关。总之，与年龄相关的主动脉僵硬度增加与主动脉壁直径增加、胶原蛋白和弹性蛋白含量减少/mm（2）、弹性层开窗增加以及胶原蛋白和弹性蛋白中荧光物质积聚有关。
During ageing and senescence the aorta becomes stiffer and its elasticity is reduced. The mechanism causing this increased stiffness of the aortic wall was studied using a rat model. Ring-shaped samples were prepared from the thoracic aorta of three groups of rats aged 4.5, 14 and 27 months, representing young, adult and old animals. Analysis of the static biomechanical properties showed increased diameter (2.20 +/- 0.03 mm) and increased stiffness (4.0 +/- 0.2 mN) of aortic samples from old rats compared with adult rats (1.82 +/- 0.02 mm and 3.0 +/- 0.1 mN, respectively). The total hydroxyproline and elastin content per sample was not changed. However, the hydroxyproline content/mm(2) of the aortic wall was reduced by 20% and the elastin content/mm(2) of the aortic wall was reduced by 19% comparing the old with the adult rats. No differences were found in the pyridinoline concentrations between old and adult rats. The collagen- and elastin-associated fluorescence was determined as a marker of advanced glycation endproducts (AGE). Both parameters were increased in the old rats compared with the adult rats, by 42% and 17%, respectively, and positively correlated with stiffness at physiological loads. A positive correlation between collagen-associated fluorescence and maximum stiffness was found as well. In conclusion, the age-related increase in stiffness of the aorta was associated with increased diameter, reduced collagen and elastin contents/mm(2) of the aortic wall, increased fenestration of elastic laminae and accumulation of fluorescent material in collagen and elastin.