Regulatory expression of Neurensin-1 in the spinal motor neurons after mouse sciatic nerve injury
Regulatory expression of Neurensin-1 in the spinal motor neurons after mouse sciatic nerve injury
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DOI：
10.1016/j.neulet.2007.03.077
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发表时间：
2007-06
影响因子：
2.5
通讯作者：
Haruno Suzuki;K. Tohyama;Kizashi Nagata;S. Taketani;M. Araki
中科院分区：
文献类型：
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作者：
Haruno Suzuki;K. Tohyama;Kizashi Nagata;S. Taketani;M. Araki
坐骨神经挤压伤后轴突再生的分子和细胞机制的阐明已被深入研究。神经突延伸因子1（Neurite extension factor1，Nrsn1）是一种独特的膜蛋白，具有微管结合结构域，在神经元中特异表达。我们的研究表明，Nrsn1是本地化，特别是在积极延伸的神经突，从而发挥作用，在膜运输到不断增长的远端延伸的神经突。为了阐明Nrsn1在外周轴突再生过程中可能发挥的作用，我们采用小鼠模型，通过原位杂交和免疫细胞化学方法研究了Nrsn1 mRNA的表达。结果显示，在运动神经轴突再生的早期阶段，Nrsn1 mRNA在受损的运动神经元中上调。Nrsn1定位于运动神经元的细胞体和再生轴突的生长远端。这些结果表明，Nrsn1在轴突再生以及胚胎发育中起着积极的作用。
Axonal regeneration after crush injury of the sciatic nerve has been intensely studied for the elucidation of molecular and cellular mechanisms. Neurite extension factor1 (Nrsn1) is a unique membranous protein that has a microtubule-binding domain and is specifically expressed in neurons. Our studies have shown that Nrsn1 is localized particularly in actively extending neurites, thus playing a role in membrane transport to the growing distal ends of extending neurites. To elucidate the possible role of Nrsn1 during peripheral axonal regeneration, we examined the expression of Nrsn1 mRNA by in situ hybridization and Nrsn1 localization by immunocytochemistry, using a mouse model. The results revealed that during the early phase of axonal regeneration of motor nerves, Nrsn1 mRNA is upregulated in the injured motor neuron. Nrsn1 is localized in the cell bodies of motor neurons and at the growing distal ends of regenerating axons. These results indicate that Nrsn1 plays an active role in axonal regeneration as well as in embryonic development.