Characterization of sarcomeric myosin heavy chain genes.
Characterization of sarcomeric myosin heavy chain genes.
复制标题
肌节肌球蛋白重链基因的表征。
DOI：
10.1016/s0021-9258(18)33307-6
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
B. Nadal
中科院分区：
文献类型：
--
作者：
R. Wydro;H. Nguyen;R. Gubits;B. Nadal
肌球蛋白重链由一个大的多基因家族编码。使用pMHC-25（一种从大鼠肌原性细胞系L6E9中分离出来的重组cDNA克隆），在大鼠中分离出了该家族的四个成员，并显示出其具有组织特异性和发育调节性。这些基因的编码区共享与非同源区域间隔的同源区域。对一种胚胎和一种成年肌球蛋白重链基因的详细分析表明，编码序列被许多插入序列打断，这些插入序列的数量、大小和分布在同一生物体或物种之间似乎并不保守。
Myosin heavy chain is encoded by a large multigene family. Using pMHC-25, a recombinant cDNA clone isolated from the rat myogenic cell line L6E9, four members of this family in the rat have been isolated and shown to be tissue-specific and developmentally regulated. The coding regions of these genes share regions of homology interspaced with regions of non-homology. Detailed analysis of one embryonic and one adult myosin heavy chain gene shows that the coding sequences are interrupted by numerous intervening sequences whose number, size, and distribution do not appear to be conserved in the same organism or between species.