Cloning, chromosomal localization, and functional expression of the alpha 1 subunit of the L-type voltage-dependent calcium channel from normal human heart.
Cloning, chromosomal localization, and functional expression of the alpha 1 subunit of the L-type voltage-dependent calcium channel from normal human heart.
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DOI：
10.1073/pnas.90.13.6228
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发表时间：
1993-07
影响因子：
11.1
通讯作者：
David Schultz;Gabor Mikala;Atsuko Yatani;Dorothy B. Engle;David E. ILESt;Bart SEGERSt;Richard J. Sinke;D. Weghuis;Udo Klockner;Minoru Wakamori;JING-JING Wang-JING;David Melvin;G. Varadi;Arnold Schwartz
中科院分区：
文献类型：
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作者：
David Schultz;Gabor Mikala;Atsuko Yatani;Dorothy B. Engle;David E. ILESt;Bart SEGERSt;Richard J. Sinke;D. Weghuis;Udo Klockner;Minoru Wakamori;JING-JING Wang-JING;David Melvin;G. Varadi;Arnold Schwartz
从正常人心脏mRNA文库中分离出心脏L型电压依赖性钙通道α 1亚基cDNA的一种独特结构变体。推导的氨基酸序列与其他钙通道α 1亚基具有显著的同源性。然而，与兔心脏α 1的差异包括缩短的N-末端，独特的C-末端插入，以及两种形式的可变剪接基序IV S3区。缩短的N-末端提供了最佳的进入共有序列的途径，被认为有助于翻译。北方印迹分析显示一个9.4 kb的杂交mRNA种类。人类心脏α 1亚基的基因特异性地定位于染色体12 p13的远端区域。克隆的α 1亚基在非洲爪蟾卵母细胞中表达，单通道分析显示了天然的药理学和通道特性。
A unique structural variant of the cardiac L-type voltage-dependent calcium channel alpha 1 subunit cDNA was isolated from libraries derived from normal human heart mRNA. The deduced amino acid sequence shows significant homology to other calcium channel alpha 1 subunits. However, differences from the rabbit heart alpha 1 include a shortened N-terminus, a unique C-terminal insertion, and both forms of an alternatively spliced motif IV S3 region. The shortened N-terminus provides optimal access to consensus sequences thought to facilitate translation. Northern blot analysis revealed a single hybridizing mRNA species of 9.4 kb. The gene for the human heart alpha 1 subunit was localized specifically to the distal region of chromosome 12p13. The cloned alpha 1 subunit was expressed in Xenopus oocytes and single-channel analyses revealed native-like pharmacology and channel properties.