Serotonin transport kinetics correlated between human platelets and brain synaptosomes.
Serotonin transport kinetics correlated between human platelets and brain synaptosomes.
复制标题
人血小板和脑突触体之间的血清素转运动力学相关。
DOI：
10.1007/s00213-005-2178-6
复制
发表时间：
2005
影响因子：
3.4
通讯作者：
Smith,Joseph
中科院分区：
文献类型：
--
作者：
Rausch,JeffreyL;Johnson,MariaE;Li,Junqing;Hutcheson,Julian;Carr,BenjaminM;Corley,KatinaM;Gowans,AmandaB;Smith,Joseph
血小板已被广泛用作研究血清素转运体（SERT）在各种精神疾病，特别是抑郁症中的作用的模型系统。然而，迄今为止，尚不清楚血小板5-羟色胺（5-HT）转运是否与脑内转运有关。目的观察5-羟色胺在人血小板和大脑皮层突触体中的转运动力学，探讨两者之间是否存在相关性。方法采集25例癫痫手术患者的血小板和突触体。从手术神经病理标本的前颞叶皮层正常边缘获得突触体。结果在控制了性别（t= - 2.4,p=0.025）和时间（t=2.1,p<0.05）的混杂效应后，血小板sertvmax与脑SERTVmaxon线性回归显著相关（r=0.58,p<0.005）。血小板5-HT转运最大速度（Vmax）与po2呈负相关（r= - 0.52,p<0.01）。女性的po2明显高于男性（F=4.9,p<0.05）。在考虑性别差异后，考虑到上述po2的性别差异，添加po2并没有进一步增强回归。血小板与脑SERTVmaxwasr=0.3,p=0.06的相关性。结论这些结果表明，在适当控制混杂因素的情况下，血小板中5-羟色胺转运与皮质突触体之间可能存在关系。
RationaleBlood platelets have been used extensively as a model system for investigating the role of the serotonin transporter (SERT) in various psychiatric disorders, especially depression. However, to date, it is not known whether platelet serotonin (5-HT) transport would be related to that in brain.ObjectivesWe examined 5-HT transport kinetics simultaneously in human blood platelets and human cortical brain synaptosomes to determine whether they were correlated.MethodsBlood platelets and synaptosomes were obtained from 25 patients undergoing epileptic surgery. Synaptosomes were obtained from normal margins of surgical neuropathology specimens of anterotemporal cortex.ResultsPlatelet SERTVmaxwas significantly correlated with brain SERTVmaxon linear regression (r=0.58,p<0.005), after controlling for the confounding effects of gender (t=−2.4,p=0.025) and time of day (t=2.1,p<0.05). Consistent with previous observations, there was a negative correlation between the maximum velocity (Vmax) of platelet 5-HT transport andpO2(r=−0.52,p<0.01). Females had a significantly higherpO2than males (F=4.9,p<0.05). After accounting for gender differences, addition ofpO2did not add further strength to the regression, given the aforementioned gender differences inpO2. The correlation between unadjusted values for platelet vs brain SERTVmaxwasr=0.3,p=0.06.ConclusionsThese results suggest that a relationship may exist between 5-HT transport in platelets and cortical synaptosomes, when appropriate controls for confounding factors are employed.