RESPONSE OF PLASMODIUM REFRACTORY AND SUSCEPTIBLE STRAINS OF ANOPHELES-GAMBIAE TO INOCULATED SEPHADEX BEADS
RESPONSE OF PLASMODIUM REFRACTORY AND SUSCEPTIBLE STRAINS OF ANOPHELES-GAMBIAE TO INOCULATED SEPHADEX BEADS
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DOI：
10.1016/0145-305x(94)90002-7
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发表时间：
1994-09-01
影响因子：
2.9
通讯作者：
RIEHLE, MA
中科院分区：
文献类型：
--
作者：
PASKEWITZ, S;RIEHLE, MA
冈比亚按蚊是一种难治性蚊子，它能用黑色素包裹并消灭中肠中的疟原虫。在密切相关的敏感菌株中观察到寄生虫的正常发育。为了检查反应差异的基础，比较了两种菌株对不同电荷的接种Sephadex珠的反应。带负电荷的C-25珠引起了更强的反应，在难治菌株，其中49%的珠强烈黑化24小时，相比之下，只有5%的敏感菌株。每个菌株的雄性蚊子的反应相似，与100%的珠强烈黑化24小时的难治菌株相比，只有5%的敏感株男性。一个时间过程显示，黑化在难治性，但不敏感的蚊子大大增加，随着时间的推移，91%的C-25珠黑化难治性女性在72小时相比，9%的易感样品。中性G-25珠粒和带正电荷的A-25珠粒在两种菌株中均被黑化，表明易感蚊子中存在黑化外来颗粒的能力。
A refractory strain of the mosquito, Anopheles gambiae, melanotically encapsulates and destroys malaria parasites in the midgut. Normal development of parasites is observed in a closely related susceptible strain. To examine the basis for the difference in response, the two strains were compared for responses to inoculated Sephadex beads of varying charge. Negatively charged C-25 beads elicited a much stronger reaction in the refractory strain where 49% of the beads were strongly melanized by 24 h, compared with only 5% in the susceptible strain. Male mosquitoes of each strain responded similarly, with 100% of the beads strongly melanized by 24 h in the refractory strain compared with only 5% in the susceptible strain males. A time course revealed that the melanization in refractory but not susceptible mosquitoes increases substantially over time; 91% of C-25 beads were melanized in refractory females at 72 h compared with 9% in the susceptible sample. Neutral G-25 beads and positively charged A-25 beads were melanized in both strains, demonstrating that the capacity to melanize foreign particles is present in susceptible mosquitoes.