PULSED-FIELD GEL-ELECTROPHORESIS OF NOTI DIGESTS OF LEPTOSPIRAL DNA - A NEW RAPID METHOD OF SEROVAR IDENTIFICATION
PULSED-FIELD GEL-ELECTROPHORESIS OF NOTI DIGESTS OF LEPTOSPIRAL DNA - A NEW RAPID METHOD OF SEROVAR IDENTIFICATION
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DOI：
10.1128/jcm.30.7.1696-1702.1992
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发表时间：
1992-07-01
影响因子：
9.4
通讯作者：
SAINTGIRONS, I
中科院分区：
文献类型：
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作者：
HERRMANN, JL;BELLENGER, E;SAINTGIRONS, I
采用脉冲场凝胶电泳（PFGE）技术对72株致病性钩端螺旋体血清型参考菌株进行NotI酶切，获得指纹图谱。这些菌株包括Australis、Ballum、Bataviae、grippo伤寒、Panama、Pomona和Pyrogenes血清群的血清型参考菌株。64个血清型可以通过唯一的NotI限制谱来识别。其余血清型通过SgrAI染色体消化进行分化。其中包括来自Australis血清组的4个血清型，来自Ballum血清组的2个血清型和来自Bataviae血清组的2个血清型。经微凝集试验和交叉吸附鉴定的18株临床分离菌中，有13株PFGE分型正确。结果表明，PFGE比血清学快速得多，可用于鉴定和流行病学研究。
Fingerprints for 72 reference serovar strains of pathogenic Leptospira spp. were obtained by pulsed-field gel electrophoresis (PFGE) following NotI restriction digests of the chromosome. These strains included the serovar reference strains of serogroups Australis, Ballum, Bataviae, Grippotyphosa, Panama, Pomona, and Pyrogenes. Sixty-four serovars could be identified by a unique NotI restriction profile. The remaining serovars were differentiated by chromosomal digestion with SgrAI. These included four serovars from serogroup Australis, two serovars from serogroup Ballum, and two serovars from serogroup Bataviae. Thirteen of 18 recent clinical isolates identified by microagglutination test and cross-adsorption procedure were correctly typed by PFGE. The results indicate that PFGE, which is considerably more rapid than serology, should be useful for identification and epidemiological studies.