Activation of LEDGF gene by thermal- and oxidative-stresses
Activation of LEDGF gene by thermal- and oxidative-stresses
复制标题
DOI：
10.1006/bbrc.2000.3606
复制
发表时间：
2000-10-05
影响因子：
3.1
通讯作者：
Shinohara, T
中科院分区：
文献类型：
--
作者：
Sharma, P;Singh, DP;Shinohara, T
LEDGF促进许多细胞类型抵抗广泛的环境应激的存活，并且响应于这些应激的细胞表达更高水平的热休克蛋白（Hsps）。LEDGF/p75是一般转录的弱共激活因子。我们推测，应激信号可能激活LEDGF基因的表达，导致LEDGF水平升高，这可能激活应激相关蛋白的表达，以保护细胞免受应激。将透镜上皮细胞（LECs）和COS 7细胞分别置于热应激和氧化应激条件下培养，培养一定时间后，用RT-PCR、北方印迹和蛋白印迹分析方法检测LEDGF mRNA和蛋白的表达。因此，应激相关保护性事件中的主要事件之一是LEDGF（一种调节蛋白）的激活，（C）2000 Academic Press.
LEDGF promotes survival of many cell types against a wide range of environmental stresses, and cells responding to those stresses expressed higher levels of heat shock proteins (Hsps). LEDGF/p75 is a weak coactivator of general transcription. We speculated that a stress signal may activate expression of the LEDGF gene, resulting in elevated levels of LEDGF which may activate expression of stress-related proteins to protect cells from stresses. Lens epithelial cells (LECs) and cos7 cells were cultured under heat- or oxidative-stress, After cells mere cultured for defined time, we quantified LEDGF mRNA and LEDGF protein with RT-PCR, Northern blot, and protein blot analysis, Our results showed that higher levels of LEDGF were found in both cell types under heat- and oxdative-stress than that in cells cultured at nonstress condition. Thus, one of the primary events in the stress-related protective events is the activation of LEDGF, a regulatory protein, (C) 2000 Academic Press.