Affinity isolation of transcriptionally active DNA.
Affinity isolation of transcriptionally active DNA.
复制标题
转录活性 DNA 的亲和分离。
DOI：
10.1016/0006-291x(86)91234-9
复制
发表时间：
1986
影响因子：
3.1
通讯作者：
Herman,T
中科院分区：
文献类型：
--
作者：
Roseman,B;Lough,J;Houkom,E;Herman,T
鸡红细胞核用化学可切割的生物素化核苷酸进行缺口翻译。BIO-12-SS-dUTP。纯化DNA，限制性内切酶消化，亲和素-琼脂糖亲和层析。70%的尼克翻译的DNA结合在柱子上。通过化学切割连接生物素和DNA的连接臂，从柱子中回收DNA。对该DNA的斑点杂交分析显示，α-D-珠蛋白基因显著丰富。这一结果表明了一种分离转录活性基因的方法。
Chicken erythrocyte nuclei were nick translated with the chemically cleavable biotinylated nucleotide. Bio-12-SS-dUTP. DNA was purified, digested with restriction endonucleases, and applied to an avidin-agarose affinity column. Seventy percent of the nick translated DNA bound to the column. This DNA was recovered from the column by chemical cleavage of the linker arm joining biotin to the DNA. Dot hybridization analysis of this DNA revealed a significant enrichment of the alpha-D-globin gene. This result suggests an approach to isolate transcriptionally active genes.