Intestinal Microbiota of Infants With Colic: Development and Specific Signatures
Intestinal Microbiota of Infants With Colic: Development and Specific Signatures
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DOI：
10.1542/peds.2012-1449
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发表时间：
2013-02-01
期刊：
影响因子：
8
通讯作者：
de Vos, Willem M.
中科院分区：
文献类型：
--
作者：
de Weerth, Carolina;Fuentes, Susana;de Vos, Willem M.
目的：为了提供一个全面的分析粪便微生物群在绞痛的婴儿，与对照组婴儿相比，在他们的第一个100天的生命。方法：微生物DNA的200多个样本，从12个绞痛的婴儿和12个年龄匹配的对照组婴儿提取和杂交到一个系统发育微阵列。而且在第一周，绞痛组的多样性显著低于对照组。在前几周有绞痛的婴儿中，连续样品的稳定性也明显较低。进一步的分析揭示了哪些细菌群在1周或2周时导致了肠绞痛相关的微生物群差异，最早的年龄具有显著差异。与对照组婴儿相比，肠绞痛婴儿的变形菌显著增加，相对丰度超过两倍。相反，肠绞痛婴儿的双歧杆菌和乳酸杆菌显著减少。此外，绞痛表型与特定的变形菌群正相关，包括与埃希氏菌属、克雷伯氏菌属、沙雷氏菌属、弧菌属、耶尔森氏菌属和假单胞菌属相关的细菌，但与属于拟杆菌门和厚壁菌门的细菌负相关，厚壁菌门包括一些乳酸杆菌和已知产生丁酸盐和乳酸盐的典型菌群。研究结果表明，在婴儿出生后的头几周内存在微生物特征，这些婴儿后来发展为绞痛。这些微生物特征可以用来理解过度哭泣。这些结果为早期诊断和开发特定疗法提供了机会。儿科2013; 131：e550-e558
OBJECTIVES: To provide a comprehensive analysis of the fecal microbiota in infants with colic, as compared with control infants, during their first 100 days of life.METHODS: Microbial DNA of >200 samples from 12 infants with colic and 12 age-matched control infants was extracted and hybridized to a phylogenetic microarray.RESULTS: Microbiota diversity gradually increased after birth only in the control group; moreover, in the first weeks, the diversity of the colic group was significantly lower than that of the control group. The stability of the successive samples also appeared to be significantly lower in the infants with colic for the first weeks. Further analyses revealed which bacterial groups were responsible for colic-related differences in microbiota at age 1 or 2 weeks, the earliest ages with significant differences. Proteobacteria were significantly increased in infants with colic compared with control infants, with a relative abundance that was more than twofold. In contrast, bifidobacteria and lactobacilli were significantly reduced in infants with colic. Moreover, the colic phenotype correlated positively with specific groups of proteobacteria, including bacteria related to Escherichia, Klebsiella, Serratia, Vibrio, Yersinia, and Pseudomonas, but negatively with bacteria belonging to the Bacteroidetes and Firmicutes phyla, the latter of which includes some lactobacilli and canonical groups known to produce butyrate and lactate.CONCLUSIONS: The results indicate the presence of microbial signatures in the first weeks of life in infants who later develop colic. These microbial signatures may be used to understand the excessive crying. The results offer opportunities for early diagnostics as well as for developing specific therapies. Pediatrics 2013; 131: e550-e558