The nuclear tyrosine kinase Rak associates with the retinoblastoma protein pRb.
The nuclear tyrosine kinase Rak associates with the retinoblastoma protein pRb.
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DOI：
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发表时间：
1995-09
期刊：
影响因子：
11.2
通讯作者：
R. Craven;W. Cance;E. Liu
中科院分区：
文献类型：
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作者：
R. Craven;W. Cance;E. Liu
Rak是一种核酪氨酸激酶，其NH2末端含有Src同源结构域2和3。我们在这里报道了视网膜母细胞瘤肿瘤易感基因产物pRb在体内和体外与Rak相关。在细胞周期的G1和S期，Rak结合在pRb的A/B口袋区，这是人类癌症中经常发生突变的区域。此外，Rak在G1期表达升高，将Rak转染到NIH 3T3细胞中可显著减少新生菌落的数量。因此，Rak是一种酪氨酸激酶，具有生长抑制活性，可能部分通过其与pRb的相互作用起作用。
Rak is a nuclear tyrosine kinase containing Src homology 2 and 3 domains at its NH2 terminus. We report here that the retinoblastoma tumor susceptibility gene product pRb associates with Rak in vivo and in vitro. Rak binds in the A/B pocket region of pRb, a region that is frequently mutated in human cancer, during the G1 and S phases of the cell cycle. Furthermore, Rak expression is elevated in G1, and transfection of Rak into NIH 3T3 cells results in a significant decrease in the number of emerging colonies. Thus, Rak is a tyrosine kinase with growth suppressing activity that may function, in part, through its interaction with pRb.